Proteomics

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DIA LC-MSMS of MCF-7 Breast Cancer Cells

ABSTRACT: The breast cancer cell line MCF-7 was treated with doxorubicin. Protein expression differences between treated and non-treated cells were semiquantitatively determined using the precursor acquisition independent from ion count (PAcIFIC) mass spectrometric method. The acquired proteomic data sets were searched for regulated Reactome pathways and Gene Ontology annotation terms using a new algorithm (SetRank). Using this approach, we described significantly changed pathways (p-value of 0.05 or less) already known to be influenced by chemotherapeutic drugs, such as chromatin organization, DNA binding, embryo development, condensed chromosome, sequence-specific DNA binding, response to oxidative stress and response to toxin. Additionally, we found pathways such as central nervous system neuron differentiation, neuron projection membrane and SNAP receptor activity explaining side-effects of doxorubicin chemotherapy, characterized as ‘chemo brain’, on a molecular level.

INSTRUMENT(S): LTQ

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Manfred Heller  

LAB HEAD: Manfred Heller

PROVIDER: PXD002998 | Pride | 2015-11-09

REPOSITORIES: Pride

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Dataset's files

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Action DRS
13Jul24_sample1_PACIFC_i01.protXML Other
13Jul24_sample1_PACIFC_i02.protXML Other
13Jul24_sample1_PACIFC_i03.protXML Other
13Jul24_sample1_PACIFC_i04.protXML Other
13Jul24_sample1_PACIFC_i05.protXML Other
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Publications

Doxorubicin Affects Expression of Proteins of Neuronal Pathways in MCF-7 Breast Cancer Cells.

Petrovic Marian M   Simillion Cedric C   Kruzliak Peter P   Sabo Jan J   Heller Manfred M  

Cancer genomics & proteomics 20151101 6

In the present article, we report on the semi-quantitative proteome analysis and related changes in protein expression of the MCF-7 breast cancer cell line following treatment with doxorubicin, using the precursor acquisition independent from ion count (PAcIFIC) mass spectrometry method. PAcIFIC represents a cost-effective and easy-to-use proteomics approach, enabling for deep proteome sequencing with minimal sample handling. The acquired proteomic data sets were searched for regulated Reactome  ...[more]

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