Dataset Information

Mouse Liver S-Palmitoylation ZDHHC13 LC-MS/MS

ABSTRACT: Protein S-palmitoylation is a reversible post-translational modification which adding 16-carbon palmitate to specific cysteines and contributes to variable biological functions such as protein trafficking, scaffolding and signaling. ZDHHC13 is one of the members of DHHC-containing palmitoyl-acyltransferase (PAT) family of enzyme. The Zdhhc13 deficient mice show the most severe phenotype with amyloidosis, alopecia, and osteoporosis. Here we used quantitative proteomic approach, combining resin-assisted capture (RAC) method and mass spectrometry-based label free strategy to identify the candidate Zdhhc13 substrates from mouse liver tissue.

INSTRUMENT(S): LTQ Orbitrap, Q-Tof Premier

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

DISEASE(S): Osteoporosis,Alopecia,Lipid Metabolism Disorder,Amyloidosis

SUBMITTER: Yi-Ju Chen

LAB HEAD: Yi-Ju Chen

PROVIDER: PXD003039 | Pride | 2017-09-29

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	130928-LiverPalmCys-allCombined.mgf	Mgf
	131218-Liverx3Palm-SH-XL-all.mgf	Mgf
	131218_liverK1HAPalmSH.mgf	Mgf
	131218_liverK1PalmSH.mgf	Mgf
	131218_liverK2HAPalmSH.mgf	Mgf

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Publications

Role of S-Palmitoylation by ZDHHC13 in Mitochondrial function and Metabolism in Liver.

Shen Li-Fen LF Chen Yi-Ju YJ Liu Kai-Ming KM Haddad Amir N Saleem ANS Song I-Wen IW Roan Hsiao-Yuh HY Chen Li-Ying LY Yen Jeffrey J Y JJY Chen Yu-Ju YJ Wu Jer-Yuarn JY Chen Yuan-Tsong YT

Scientific reports 20170519 1

Palmitoyltransferase (PAT) catalyses protein S-palmitoylation which adds 16-carbon palmitate to specific cysteines and contributes to various biological functions. We previously reported that in mice, deficiency of Zdhhc13, a member of the PAT family, causes severe phenotypes including amyloidosis, alopecia, and osteoporosis. Here, we show that Zdhhc13 deficiency results in abnormal liver function, lipid abnormalities, and hypermetabolism. To elucidate the molecular mechanisms underlying these d ...[more]

PMID: 28526873

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