Proteomics

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Immunoproteomic analysis of antibody responses to extracellular proteins of Candida albicans in serum of candidemia patients revealed glycosylation effects on protein antigen recognition


ABSTRACT: During infection the yeast Candida albicans undergoes a yeast-to-hyphal transition and secretes numerous proteins required for the invasion and damage of host tissues and the modulation of the host immune responses. Still little is known about the interplay of Candida albicans secreted proteins and the host adaptive immune system. For the characterization of the extracellular proteins of C. albicans and their ability to trigger a serological response in candidemia patients we applied 2D gel electrophoresis and LC-MS/MS-based approaches. These analyses identified the secretion of 101 different proteins from C. albicans yeast cells and 410 proteins from hyphal cells. Morphology-dependent changes of the secretome were mostly associated with proteins involved in cell wall organization, general stress response and the interplay with host immune cells. Our immunoproteomics workflow for the identification of secreted C. albicans protein antigens revealed a core set of 20 immunodominant specific anti- C. albicans protein antibodies. However, some secreted protein antigens showed cross-reactivity with sera from control groups without Candida infection. Glycoprotein staining of C. albicans hyphal secretome demonstrated that some secreted proteins are strongly glycosylated. Enzymatic deglycosylation of secreted proteins uncovered a significant contribution of glycan epitopes to the recognition of the secreted aspartyl protease Sap6 by IgG antibodies from patient sera.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Candida Albicans (yeast)

SUBMITTER: Thomas Krueger  

LAB HEAD: Olaf Kniemeyer

PROVIDER: PXD003201 | Pride | 2022-03-03

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
HyphaeBR1a.mgf Mgf
HyphaeBR1a.mzid.gz Mzid
HyphaeBR1a.raw Raw
HyphaeBR1b.mgf Mgf
HyphaeBR1b.mzid.gz Mzid
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