Proteomics

Dataset Information

0

Proteomics of Extracellular Vesicles Harvested with ExtraPEG

ABSTRACT: Polyethylene glycol (PEG) is commonly used to isolate virus particles from culture medium. This project adapted and optimized the virus procedure for harvesting extracellular vesicles (of similar biophysical properties to viruses) from media. An 8% PEG solution harvested the purest vesicles. Followed by a wash step (ultracentrifugation of concentrated vesicles in saline), this method produced preparations comparable or superior to commonly used methods in terms of purity, abundance, and cost. It is also highly adaptable, allowing the procedure to be used for enriching vesicles from a variety of biological fluids. As proof of principle, extracellular vesicle protein was successfully harvested from HeLa cell medium using the described PEG-based harvest method and analyzed by mass spectrophotometry.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Epithelial Cell, Cell Culture

SUBMITTER: Mark Rider  

LAB HEAD: David G. Meckes Jr.

PROVIDER: PXD003209 | Pride | 2016-04-18

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
01112016_DM798_Run1-01.msf Msf
01112016_DM798_Run1-01.mzid.gz Mzid
01112016_DM798_Run1-01.mzid_01112016_DM798_Run1-01.MGF Mzid
01112016_DM798_Run1-01.mzid_01112016_DM798_Run1-01.pride.mgf.gz Mzid
01112016_DM798_Run1-01.pride.mztab.gz Mztab
Items per page:
1 - 5 of 19
altmetric image

Publications

ExtraPEG: A Polyethylene Glycol-Based Method for Enrichment of Extracellular Vesicles.

Rider Mark A MA   Hurwitz Stephanie N SN   Meckes David G DG  

Scientific reports 20160412

Initially thought to be a means for cells to eliminate waste, secreted extracellular vesicles, known as exosomes, are now understood to mediate numerous healthy and pathological processes. Though abundant in biological fluids, purifying exosomes has been challenging because their biophysical properties overlap with other secreted cell products. Easy-to-use commercial kits for harvesting exosomes are now widely used, but the relative low-purity and high-cost of the preparations restricts their ut  ...[more]

Similar Datasets

Proteomics Proteomics of Extracellular Vesicles Harvested with ExtraPEG
2016-07-22 | MSV000079996 | MassIVE
Transcriptomics CELL-EXO_01
2013-07-23 | E-MEXP-3740 | biostudies-arrayexpress
Transcriptomics Proteomic signatures of extracellular vesicles secreted by non-mineralizing and mineralizing human osteoblasts and stimulation of tumor cell growth
2014-10-28 | E-GEOD-56172 | biostudies-arrayexpress
Transcriptomics RNA and miRNA sequencing of extracellular vesicles derived from murine Th1 and Th2 differentiated cells
2022-05-06 | E-MTAB-8823 | biostudies-arrayexpress
Transcriptomics mRNA expression of brain endothelial cells
2015-04-02 | E-GEOD-63447 | biostudies-arrayexpress
Transcriptomics miRNA expression of extracellular vesicles
2015-04-02 | E-GEOD-63445 | biostudies-arrayexpress
Proteomics Proteomic profile of membrane vesicles of E. faecium
2018-06-10 | PXD008801 | Pride
Transcriptomics miRNA content in extracellular vesicles
2021-08-07 | E-MTAB-10858 | biostudies-arrayexpress
Transcriptomics Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles
2014-07-15 | E-GEOD-54111 | biostudies-arrayexpress
Proteomics Vesicle proteomics during fungal biofilm development in Candida
2022-10-14 | PXD035068 | Pride