Proteomics

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Proteomics assisted studies of the host nuclear proteome identifies candidate regulators of immunity modified during Phytophthora capsici-tomato interactions


ABSTRACT: In this study we have combined nuclei enrichment with quantitative proteomics to study tomato nuclear proteome dynamics during early infection with the devastating broad host-range oomycete pathogen Phytophthora capsici. Using this method we have identified hundreds of host nuclear proteins that show specific changes in abundance during P. capsici infection including proteins implicated in nucleic acid binding, oxidoreductase activity, helicase activity, RNA binding and transcription regulator activity. A number of these proteins have known links to plant immunity or defence signalling. We have confirmed the nuclear localisation of a selection of proteins which show P. capsici infection dependant changes in abundance and have examined the impact of their expression on resistance. As direct proof of biological relevance we demonstrate that in planta overexpression of an AT-hook family protein which decreased in abundance during infection, causes enhanced resistance to P. capsici. We conclude that organelle enrichment combined with quantitative proteomics represents a valuable strategy, suited to interrogate dynamic proteomes during stress, and allows the identification of promising targets to bolster immunity in crops.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Andrew Howden  

LAB HEAD: Dr Edgar Huitema

PROVIDER: PXD003260 | Pride | 2017-04-18

REPOSITORIES: Pride

Dataset's files

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Action DRS
PTSS3882SN14693.raw Raw
PTSS3882SN14694.raw Raw
PTSS3882SN14695.raw Raw
PTSS3882SN14696.raw Raw
PTSS3882SN14697.raw Raw
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Publications

Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity.

Howden Andrew J M AJM   Stam Remco R   Martinez Heredia Victor V   Motion Graham B GB   Ten Have Sara S   Hodge Kelly K   Marques Monteiro Amaro Tiago M TM   Huitema Edgar E  

The New phytologist 20170410 1


Plant-pathogen interactions are complex associations driven by the interplay of host and microbe-encoded factors. With secreted pathogen proteins (effectors) and immune signalling components found in the plant nucleus, this compartment is a battleground where susceptibility is specified. We hypothesized that, by defining changes in the nuclear proteome during infection, we can pinpoint vital components required for immunity or susceptibility. We tested this hypothesis by documenting dynamic chan  ...[more]

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