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Growth-phase regulated acetylation in B. subtilis

ABSTRACT: To uncover potential regulatory roles for acetylation, we analyzed how acetylation patterns and abundances change between logarithmic and stationary phase growth in Bacillus subtilis. Using acetyllysine peptide enrichment followed by label-free mass spectrometry-based proteomics, we identified over 2,300 unique acetylation sites on proteins that function in many essential pathways. By comparing the changes in acetylation with total cell protein abundances, we discover a subset of specific acetylation events that are stoichiometrically regulated under different growth conditions.

INSTRUMENT(S): LTQ Orbitrap, LTQ Orbitrap Velos

ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. 168

SUBMITTER: Todd Greco

LAB HEAD: Ileana Cristea

PROVIDER: PXD003272 | Pride | 2016-07-07

REPOSITORIES: pride

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			Action	DRS
	2013_07_19_ac_1x_CID.raw	Raw
	2013_07_19_ac_1x_HCD.raw	Raw
	2014-08-01-acetyl-MIN-log_CID.raw	Raw
	2014-08-01-acetyl-MIN-log_HCD.raw	Raw
	2014-08-21-acetyl-MIN-log_CID.raw	Raw

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Publications

Temporal Regulation of the Bacillus subtilis Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth.

Carabetta Valerie J VJ Greco Todd M TM Tanner Andrew W AW Cristea Ileana M IM Dubnau David D

mSystems 20160531 3

Nε-Lysine acetylation has been recognized as a ubiquitous regulatory posttranslational modification that influences a variety of important biological processes in eukaryotic cells. Recently, it has been realized that acetylation is also prevalent in bacteria. Bacteria contain hundreds of acetylated proteins, with functions affecting diverse cellular pathways. Still, little is known about the regulation or biological relevance of nearly all of these modifications. Here we charac ...[more]

PMID: 27376153

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A synthetic non-histone substrate to study substrate targeting by the Gcn5 HAT and sirtuin HDACs

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2019-02-25 | PXD012608 | Pride

Genome-wide analysis reveals determinants of redundant and specific binding within a transcription factor family

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Characterization of the acetylome associated with rapamycin-induced autophagy in HeLa cells

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