Dataset Information

Half decimal place rule

ABSTRACT: Many MS2 spectra in bottom-up proteomics experiments remain unassigned. To improve proteome coverage, we applied the half decimal place rule (HDPR) to remove non-peptidic molecules. The HDPR considers the ratio of the first digit after the decimal point to the full molecular mass and results in a relatively small permitted mass window for most peptides. Although the HDPR has been described previously, it has never been integrated into an acquisition strategy using high resolution mass spectrometers. The HDPR was applied to three technical replicates of an in-solution tryptic digest of HeLa cells which were analysed by LC-MS using a Q Exactive mass spectrometer.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Christian Koehler

LAB HEAD: Bernd Thiede

PROVIDER: PXD003308 | Pride | 2022-02-28

REPOSITORIES: pride

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Application of the half decimal place rule to increase the peptide identification rate.

Koehler Christian J CJ Bollineni Ravi Chand RC Thiede Bernd B

Rapid communications in mass spectrometry : RCM 20170101 2

<h4>Rationale</h4>Many MS2 spectra in bottom-up proteomics experiments remain unassigned. To improve proteome coverage, we applied the half decimal place rule (HDPR) to remove non-peptidic molecules. The HDPR considers the ratio of the digits after the decimal point to the full molecular mass and results in a relatively small permitted mass window for most peptides.<h4>Methods</h4>First, the HDPR mass filter was calculated for the human and other proteomes. Subsequently, the HDPR was applied to ...[more]

PMID: 27806443

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			Action	DRS
	HDPR_0.15Da_Hela_1.dat	Other
	HDPR_0.15Da_Hela_1.mgf	Mgf
	HDPR_0.15Da_Hela_2.dat	Other
	HDPR_0.15Da_Hela_2.mgf	Mgf
	HDPR_0.15Da_Hela_3.dat	Other