Dataset Information

Blanc Du Bios Proteome - Proteome Profile of American Hybrid Grape cv

ABSTRACT: Blanc Du bois grapes are gaining popularity in the South eastern US due to its distinctive flavor and disease tolerance characteristics. Berry composition at harvest is a major contributing factor of wine quality. Blanc Du bois grapes are harvested from EL-38 and EL-39 stages depending on the style of wine desired or harvested early to avoid rain nearing harvest. In the current study, gel-free proteome analysis was applied to investigate changes in enzymes, primary and secondary metabolism proteins during ripening and late ripe stage. Grape berries from EL-33, EL-34, EL-36, EL-38 and EL-39 were collected based on brix, acidity and density. Protein extracts from different berry stages were resolved by electrophoresis. Proteins were extracted from the gel as a single band, detained and subjected to proteolysis with sequencing grade trypsin. Trypsin digested peptides from different berry protein extracts were separated on a nano LC and the eluent was sprayed onto to a LTQ Orbitrap Velos mass spectrometer. The raw files were analyzed using Proteome Discoverer with Sequest and Mascot search nodes using Vitis species FASTA database (70,263 entries) and the data were further validated by Scaffold software. A total of 1091, 1131, 1078, 1042 and 1066 proteins were detected in EL-33, EL-34, EL-36, EL-38 and EL-39 of berries respectively. Statistical ANOVA analysis revealed 927 proteins present across the stages that are involved in various biochemical and metabolic pathways. Seventeen proteins including dihydroflavonol reductase, sucrose phosphate synthase, PR proteins increased more than three-fold between ripe and late ripe berry stages. Other proteins that increased during ripe and late ripe stage berries were alcohol dehydrogenase 1, anthocyanidin reductase, phospho-2-dehydro-3-deoxyheptonate aldolase, fatty acid hydroperoxide lyase, cinnamyl alcohol dehydrogenase, -isopiperitenol (-)-carveol and SAM-methyltransferases.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Vitis Vinifera (grape)

TISSUE(S): Plant Cell

SUBMITTER: Devaiah Kambiranda

LAB HEAD: Mehboob Sheikh

PROVIDER: PXD004157 | Pride | 2018-04-09

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	11042014DK_B14A_Run1.mzid	Mzid
	11042014DK_B14A_Run1.mzid_11042014DK_B14A_Run1.MGF	Mzid
	11042014DK_B14A_Run1.raw	Raw
	11042014DK_B14A_Run2.mzid	Mzid
	11042014DK_B14A_Run2.mzid_11042014DK_B14A_Run2.MGF	Mzid

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Publications

Proteome Profile of American Hybrid Grape cv. Blanc du Bois during Ripening Reveals Proteins Associated with Flavor Volatiles and Ethylene Production.

Kambiranda Devaiah D Basha Sheikh M SM Singh Rakesh R Snowden Janana J Mercer Roger R

Proteomics 20180325 8

The study of key control points in ripening is essential to improve grape wine quality. Molecular basis of ripening is still far from being understood from the Pierce's disease (PD)-tolerant grapes predominantly grown in the southeastern United States. To identify proteins expressed during Blanc du Bois grape berry green and ripening stages, proteome analysis from five different stages revealed 1091, 1131, 1078, 1042, and 1066 proteins. Differential expression analysis revealed 551 common protei ...[more]

PMID: 29359857

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