Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)
TISSUE(S): Cell Culture
SUBMITTER: Guijie Zhu
LAB HEAD: Norman Dovichi
PROVIDER: PXD003462 | Pride | 2016-07-20
REPOSITORIES: pride
Biotechnology and bioengineering 20160428 10
We employed UPLC-MS/MS with iTRAQ 8-plex labeling to quantitatively analyze the supernatant produced by two Chinese hamster ovary (CHO) cell lines (CHO K1SV and CHO CAT-S). In each case, the supernatant from the host and three transfected clones were analyzed at days 5, 7, and 10 of culture. A total of eight iTRAQ 8-plex experiments were performed. For each cell line, the overlap of supernatant protein identifications between transfected clones is over 60%. Over 70% of the supernatant proteins i ...[more]