Proteomics

Dataset Information

0

IEF fractionation OVCAR-5 cell line


ABSTRACT: Analysis of the ovarian cancer cell line OVCAR-5. A standard trypsin digest was carried out on the OVCAR-5 cell lysates which were then analysed in the un-fractionated and fractionated forms. Fractionation was completed using a peptide IEF separation method. All samples were analysed by nano-LC-ESI-MS/MS using a QTOF.

INSTRUMENT(S): maXis

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Malignant Neoplasm Of Ovary

SUBMITTER: Georgia Arentz  

LAB HEAD: Peter Hoffmann

PROVIDER: PXD003547 | Pride | 2016-09-27

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
IEF_1.baf Other
IEF_10.baf Other
IEF_11.baf Other
IEF_12.baf Other
IEF_13.baf Other
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1 - 5 of 22
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Publications

Novel IEF Peptide Fractionation Method Reveals a Detailed Profile of N-Terminal Acetylation in Chemotherapy-Responsive and -Resistant Ovarian Cancer Cells.

Weiland Florian F   Arentz Georgia G   Klingler-Hoffmann Manuela M   McCarthy Peter P   Lokman Noor A NA   Kaur Gurjeet G   Oehler Martin K MK   Hoffmann Peter P  

Journal of proteome research 20160919 11


Although acetylation is regarded as a common protein modification, a detailed proteome-wide profile of this post-translational modification may reveal important biological insight regarding differential acetylation of individual proteins. Here we optimized a novel peptide IEF fractionation method for use prior to LC-MS/MS analysis to obtain a more in depth coverage of N-terminally acetylated proteins from complex samples. Application of the method to the analysis of the serous ovarian cancer cel  ...[more]

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