Proteomics

Dataset Information

0

A human endogenous retrovirus encoded protease potentially cleaves numerous cellular proteins


ABSTRACT: The human genome consists of considerable portions derived from retroviruses typically inherited already for millions of years. So-called human endogenous retroviruses (HERVs) are usually severely mutated, yet some coding-competent HERV sequences exist. The HERV-K(HML-2) group includes evolutionarily young proviruses that still encode typical retroviral proteins. HERV-K(HML-2) has been implicated in various human diseases because transcription is often upregulated and encoded proteins are known to affect cell biology. HERV-K(HML-2) protease has received little attention so far. With findings for Human Immunodeficiency Virus (HIV) protease in mind we set out to identify human cellular proteins being substrates of HERV-K(HML-2) protease employing a modified Terminal Amine Isotopic Labeling of Substrates (TAILS) procedure. Thousands of significantly processed human proteins were revealed by TAILS and we could verify cleavage of a majority of selected human proteins in vitro. Our analysis suggests that hundreds, if not thousands of cellular proteins are potential substrates of HERV-K(HML-2) protease. As identified proteins participate in diverse, often disease-relevant cellular processes, it is conceivable that expression of HERV-K(HML-2) protease has functional consequences for cell biology and thus development of human diseases. Endogenous retrovirus-encoded protease may also be relevant for disease development in species other than human.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Oliver Schilling  

LAB HEAD: Oliver Schilling

PROVIDER: PXD010159 | Pride | 2019-09-02

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
BM3576.mzXML Mzxml
BM3576.raw Raw
BM3577.mzXML Mzxml
BM3577.raw Raw
BM3578.mzXML Mzxml
Items per page:
1 - 5 of 50
altmetric image

Publications

A human endogenous retrovirus encoded protease potentially cleaves numerous cellular proteins.

Rigogliuso Giuseppe G   Biniossek Martin L ML   Goodier John L JL   Mayer Bettina B   Pereira Gavin C GC   Schilling Oliver O   Meese Eckart E   Mayer Jens J  

Mobile DNA 20190822


<h4>Background</h4>A considerable portion of the human genome derives from retroviruses inherited over millions of years. Human endogenous retroviruses (HERVs) are usually severely mutated, yet some coding-competent HERVs exist. The HERV-K(HML-2) group includes evolutionarily young proviruses that encode typical retroviral proteins. HERV-K(HML-2) has been implicated in various human diseases because transcription is often upregulated and some of its encoded proteins are known to affect cell biol  ...[more]

Similar Datasets

2019-09-02 | PXD013296 | Pride
2023-11-03 | PXD028320 | Pride
2018-12-19 | PXD004757 | Pride
2019-04-18 | PXD010816 | Pride
2021-11-03 | PXD026797 | Pride
2020-11-10 | E-MTAB-8537 | biostudies-arrayexpress
2021-11-03 | PXD026815 | Pride
2021-11-25 | PXD028117 | Pride
2024-08-10 | PXD050580 | Pride
2015-10-19 | PXD002474 | Pride