Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos, Bruker Daltonics maXis series, Q Exactive
ORGANISM(S): Homo Sapiens (human) Escherichia Coli
SUBMITTER: Ana Martinez
LAB HEAD: Javier Muñoz Peralta
PROVIDER: PXD003640 | Pride | 2016-08-08
REPOSITORIES: Pride
Items per page: 1 - 5 of 56 |
Journal of proteome research 20160804 9
Isobaric labeling is gaining popularity in proteomics due to its multiplexing capacity. However, copeptide fragmentation introduces a bias that undermines its accuracy. Several strategies have been shown to partially and, in some cases, completely solve this issue. However, it is still not clear how ratio compression affects the ability to identify a protein's change of abundance as statistically significant. Here, by using the "two proteomes" approach (E. coli lysates with fixed 2.5 ratios in t ...[more]