Proteomics

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A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)


ABSTRACT: Improvement of freezing tolerance of red clover (Trifolium pratense L.) would increase its persistence under cold climate. In this study, we assessed the freezing tolerance and compared the proteome composition of non-acclimated and cold-acclimated plants of two initial cultivars of red clover: Endure (E-TF0) and Christie (C-TF0) and of populations issued from these cultivars after three (TF3) and four (TF4) cycles of phenotypic recurrent selection for superior freezing tolerance. Through this approach, we wanted to identify proteins that are associated with the improvement of freezing tolerance in red clover. Recurrent selection performed indoor is an effective approach to improve the freezing tolerance of red clover. Significant improvement of freezing tolerance by recurrent selection was associated with differential accumulation of a small number of cold-regulated proteins that may play an important role in the determination of the level of freezing tolerance.

INSTRUMENT(S): 4800 Proteomics Analyzer

ORGANISM(S): Trifolium Pratense Subsp. Pratense

SUBMITTER: Jenny Renaut  

LAB HEAD: Jenny Renaut

PROVIDER: PXD003689 | Pride | 2016-03-15

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20140619Trefle40733_run6-7.mzML Mzml
20140620Trefle40728.mzML Mzml
40728.zip Other
40733.zip Other
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Publications

A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.).

Bertrand Annick A   Bipfubusa Marie M   Castonguay Yves Y   Rocher Solen S   Szopinska-Morawska Aleksandra A   Papadopoulos Yousef Y   Renaut Jenny J  

BMC plant biology 20160310


<h4>Background</h4>Improvement of freezing tolerance of red clover (Trifolium pratense L.) would increase its persistence under cold climate. In this study, we assessed the freezing tolerance and compared the proteome composition of non-acclimated and cold-acclimated plants of two initial cultivars of red clover: Endure (E-TF0) and Christie (C-TF0) and of populations issued from these cultivars after three (TF3) and four (TF4) cycles of phenotypic recurrent selection for superior freezing tolera  ...[more]

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