Dataset Information

Impact of moderate temperature changes on Neisseria meningitidis adhesive phenotypes and proteome

ABSTRACT: Neisseria meningitidis, the meningococcus, bears the potential of life-threatening invasive diseases, but it usually colonizes the nasopharynx without causing any symptoms. Within the nasopharynx, Neisseria meningitidis must face temperature changes depending on the ambient air temperature. Indeed, the nasopharyngeal temperature can be substantially lower than 37°C, the temperature commonly used in experimental settings. Here, we compared meningococcal biofilm formation, autoaggregation and cellular adherence between 32°C and 37°C and found a clear increase in all these phenotypes at 32°C, suggestive for a stronger in vivo colonization capability at this temperature. A comparative proteome analysis approach revealed differential protein expression levels between 32°C and 37°C, predominantly affecting the outer membrane. Among 375 analyzed proteins, 75 were localized in the outer membrane, 37 in the periplasm, 48 in the inner membrane and 215 in the cytosol. The outer membrane proteins NHBA, NMB1030 and ACP showed strongest upregulation at 32°C and were partially responsible for the observed temperature-dependent phenotypes. Screening of different global regulators of Neisseria meningitidis revealed that the extracytoplasmic sigma factor, E, might be involved in the temperature-dependent biofilm formation. In conclusion, subtle temperature changes trigger adaptation events promoting mucosal colonization by meningococci

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Neisseria Meningitidis Serogroup B (strain Mc58)

SUBMITTER: Andreas Otto

LAB HEAD: Andreas Otto

PROVIDER: PXD003690 | Pride | 2016-10-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	130212_O2_P1_SG_Ml_37-1_1.RAW	Raw
	130212_O2_P1_SG_Ml_37-1_10.RAW	Raw
	130212_O2_P1_SG_Ml_37-1_2.RAW	Raw
	130212_O2_P1_SG_Ml_37-1_3.RAW	Raw
	130212_O2_P1_SG_Ml_37-1_4.RAW	Raw

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Publications

Impact of Moderate Temperature Changes on Neisseria meningitidis Adhesion Phenotypes and Proteome.

Lappann Martin M Otto Andreas A Brauer Madita M Becher Dörte D Vogel Ulrich U Johswich Kay K

Infection and immunity 20161118 12

Neisseria meningitidis, the meningococcus, bears the potential to cause life-threatening invasive diseases, but it usually colonizes the nasopharynx without causing any symptoms. Within the nasopharynx, Neisseria meningitidis must face temperature changes depending on the ambient air temperature. Indeed, the nasopharyngeal temperature can be substantially lower than 37°C, the temperature commonly used in experimental settings. Here, we compared the levels of meningococcal biofilm formation, auto ...[more]

PMID: 27672084

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Project description:Baart2007 - Genome-scale metabolic network of Neisseria meningitidis (iGB555) This model is described in the article: Modeling Neisseria meningitidis metabolism: from genome to metabolic fluxes. Baart GJ, Zomer B, de Haan A, van der Pol LA, Beuvery EC, Tramper J, Martens DE. Genome Biol. 2007; 8(7): R136 Abstract: BACKGROUND: Neisseria meningitidis is a human pathogen that can infect diverse sites within the human host. The major diseases caused by N. meningitidis are responsible for death and disability, especially in young infants. In general, most of the recent work on N. meningitidis focuses on potential antigens and their functions, immunogenicity, and pathogenicity mechanisms. Very little work has been carried out on Neisseria primary metabolism over the past 25 years. RESULTS: Using the genomic database of N. meningitidis serogroup B together with biochemical and physiological information in the literature we constructed a genome-scale flux model for the primary metabolism of N. meningitidis. The validity of a simplified metabolic network derived from the genome-scale metabolic network was checked using flux-balance analysis in chemostat cultures. Several useful predictions were obtained from in silico experiments, including substrate preference. A minimal medium for growth of N. meningitidis was designed and tested successfully in batch and chemostat cultures. CONCLUSION: The verified metabolic model describes the primary metabolism of N. meningitidis in a chemostat in steady state. The genome-scale model is valuable because it offers a framework to study N. meningitidis metabolism as a whole, or certain aspects of it, and it can also be used for the purpose of vaccine process development (for example, the design of growth media). The flux distribution of the main metabolic pathways (that is, the pentose phosphate pathway and the Entner-Douderoff pathway) indicates that the major part of pyruvate (69%) is synthesized through the ED-cleavage, a finding that is in good agreement with literature. This model is hosted on BioModels Database and identified by: MODEL1507180069. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.