Proteomics

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Proteome and phophoproteome analysis of distinct dendritic cell maturation states


ABSTRACT: Depending on their environment and their exposure to various factors, dendritic cells (DCs) exist in different activation and maturation states. The versatility of DCs allows them to shape the immune system towards an inflammatory or tolerant state depending on the antigens and the environment they encounter. In this study we aimed to provide a proteomic catalogue of differentially expressed and differentially phosphorylated proteins between distinct DC maturation states, brought about by bacteria that differ in their endotoxicity. To achieve this, we have performed unlabeled shotgun proteomics and phosphoproteomics on cell fractions obtained from murine bone marrow DC cultures. The symbiont B. vulgatus stimulation was used to obtain semi-mature DCs, and the pathobiont E. coli stimulation was used to obtain mature DCs. For both shotgun proteome and phosphoproteome analysis two biological replicates consisting of 8 mice were used. Each biological replicate were run as 3 technical replicates.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Dendritic Cell, Cell Culture

SUBMITTER: Marius Codrea  

LAB HEAD: Prof. Dr. med. Julia-Stefanie Frick

PROVIDER: PXD003738 | Pride | 2017-11-27

REPOSITORIES: Pride

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Publications

Proteome and phosphoproteome analysis of commensally induced dendritic cell maturation states.

Korkmaz Ali Giray AG   Popov Todor T   Peisl Loulou L   Codrea Marius Cosmin MC   Nahnsen Sven S   Steimle Alexander A   Velic Ana A   Macek Boris B   von Bergen Martin M   Bernhardt Joerg J   Frick Julia-Stefanie JS  

Journal of proteomics 20171115


Dendritic cells (DCs) can shape the immune system towards an inflammatory or tolerant state depending on the bacterial antigens and the environment they encounter. In this study we provide a proteomic catalogue of differentially expressed proteins between distinct DC maturation states, brought about by bacteria that differ in their endotoxicity. To achieve this, we have performed proteomics and phosphoproteomics on murine DC cultures. Symbiont and pathobiont bacteria were used to direct dendriti  ...[more]

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