Dataset Information

Botrytis cinerea phosphoproteome.

ABSTRACT: The phytopathogen Botrytis cinerea is a ubiquitous fungus with a high capacity to adapt its metabolism to different hosts and environmental conditions in order to deploy a variety of virulence and pathogenicity factors and develop a successful plant infection. Here we report the first comparative phosphoproteomic study of B. cinerea, aimed to analyze the phosphoprotein composition of the fungus and its changes under different phenotypical conditions induced by two different carbon sources as plant based elicitors: glucose and deproteinized tomato cell wall (TCW). A total of 2854 and 2269 different phosphosites (2883 and 1137 phosphopeptides) were identified in glucose and TCW respectively, which map to 1338 phosphoproteins in glucose and 733 in TCW. Out of the identified phosphoproteins, 173 were exclusively found when glucose was the only carbon source and 11 when the carbon source was TCW. Differences in the pattern of phosphorylation-sites were also detected according to the carbon source. Gene ontology classification of the identified phosphoproteins showed that most of the characteristic proteins of the different carbon sources were related to signaling and transmembrane transport, thus highlighting the importance of these processes in the fungal adaptation to the surrounding conditions.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Botryotinia Fuckeliana (noble Rot Fungus) (botrytis Cinerea)

SUBMITTER: Cristina Chiva

LAB HEAD: Francisco Javier Fernández Acero

PROVIDER: PXD003753 | Pride | 2016-04-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	140409_S_FFJC_01_03_PproteomaIII_45pto_rep.msf	Msf
	140409_S_FFJC_01_03_PproteomaIII_45pto_rep.raw	Raw
	140409_S_FFJC_02_03_PproteomaIII_45pto.msf	Msf
	140409_S_FFJC_02_03_PproteomaIII_45pto.raw	Raw
	140409_S_FFJC_03_03_PproteomaIII_45pto.msf	Msf

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Publications

Phosphoproteome analysis of B. cinerea in response to different plant-based elicitors.

Liñeiro Eva E Chiva Cristina C Cantoral Jesús M JM Sabido Eduard E Fernández-Acero Francisco Javier FJ

Journal of proteomics 20160318

The phytopathogen Botrytis cinerea is a ubiquitous fungus with a high capacity to adapt its metabolism to different hosts and environmental conditions in order to deploy a variety of virulence and pathogenicity factors and develop a successful plant infection. Here we report the first comparative phosphoproteomic study of B. cinerea, aimed to analyze the phosphoprotein composition of the fungus and its changes under different phenotypical conditions induced by two different carbon sources as pla ...[more]

PMID: 27003611

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Project description:Extracellular vesicles (EVs) are membranous particles released by different organisms. EVs carry several kinds of macromolecules implicated in cell communication. EVs have become an important topic in the study of pathogenic fungi, due to their relation with the fungal-host interactions. One of the essential research areas in this field is the characterization protein profile of EV cargo, because of plant fungal pathogens rely heavily on secreted proteins to invade their hosts. However, little is known about EVs of Botrytis cinerea, which is one of the most devastating phytopathogenic fungi. The present study has two main objectives: the first isolation and characterization of B. cinerea EVs; and the description of their potential role during the infective process. All the experimental process was conducted in B. cinerea growing in mimimal salt medium supplemented with glucose as a constitutive stage, and deproteinized tomato cell walls (TCW) as virulence inductor. The isolation of EV was performed by differential centrifugation, filtration, ultrafiltration, and sucrose cushion ultracentrifugation. EV fractions were visualised by TEM using negative staining. Proteomic analysis of EV cargo was addressed by LC-MS/MS. The used methodology had allowed, for the first time, the correct isolation of B. cinerea EVs, and the identification of a high number of EV proteins, including potential EV markers. The isolated EVs displayed differences in the morphology under both assayed conditions. GO analysis of EV proteins showed an enrichment in cell wall metabolism and proteolysis under TCW. KEGG analysis also showed difference in EV function under both conditions, highlighting the presence of potential virulence/pathogenic factors implicated in cell wall metabolism, between others. This is the first evidence and characterization of B. cinerea EVs production, showing that EVs are essential in the infection process of B. cinerea and share crucial functions with the conventional secretion pathway.