Dataset Information

Modifications of fungal membrane proteins profile under pathogenicity induction: a proteomic analysis of Botrytis cinerea membranome.

ABSTRACT: Botrytis cinerea is a model phytopathogenic fungus that infects more than 200 different relevant crops, thus being classified among the top ten fungal plant diseases. B. cinerea exhibits a wide arsenal of tools to infect plant tissues. Most of these factors are related to signal transduction cascades, in which membrane proteins play a key role as a bridge between environmental conditions and intracellular molecular processes. This work describes the first proteomic approach to study the membranome of B. cinerea under different pathogenicity condition induced by using different plant-based elicitors: Glucose and Tomato Cell Wall (TCW). A discovery proteomics analysis of membrane protein extracts was carried out by mass spectrometry. A total of 2,794 proteins were successfully identified, and approximately 46% of them could be classified as membrane proteins based on the presence of transmembrane regions and covalent post-translational modifications. Further analyses showed significant differences in the fungal membranome composition depending on the available carbon source: 804 proteins were exclusively identified when the fungus was cultured in the presence of glucose as sole carbon source, and 251 proteins were exclusively identified in the presence of TCW. Besides, among the 1737 common proteins, a subset of 898 proteins presented clear differences in their abundance and in order to clarify the specific changes produced in the membranome, proteins were categorized according to their gene ontology annotation shown different profiles. As a result of this work, we present here the first description of the membranome of B. cinerea, which will certainly provide a better understanding of this important subproteome in B. cinerea.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Capsaspora Owczarzaki Botryotinia Fuckeliana (noble Rot Fungus) (botrytis Cinerea)

SUBMITTER: Cristina Chiva

LAB HEAD: Francisco Javier Fernández-Acero

PROVIDER: PXD003099 | Pride | 2016-07-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	140409_FFJC_09_01_2ug.msf	Msf
	140409_FFJC_09_01_2ug.raw	Raw
	140409_FFJC_09_02_2ug.msf	Msf
	140409_FFJC_09_02_2ug.raw	Raw
	140409_FFJC_10_01_2ug.msf	Msf

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Modifications of fungal membrane proteins profile under pathogenicity induction: A proteomic analysis of Botrytis cinerea membranome.

Liñeiro Eva E Chiva Cristina C Cantoral Jesús M JM Sabidó Eduard E Fernández-Acero Francisco Javier FJ

Proteomics 20160805 17

Botrytis cinerea is a model fungus for the study of phytopathogenicity that exhibits a wide arsenal of tools to infect plant tissues. Most of these factors are related to signal transduction cascades, in which membrane proteins play a key role as a bridge between environment and intracellular molecular processes. This work describes the first description of the membranome of Botrytis under different pathogenicity conditions induced by different plant-based elicitors: glucose and tomato cell wall ...[more]

PMID: 27329576

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Project description:Transcriptomic analysis in response to Botrytis cinerea infections under conrasting nitrate regime Nitrogen (N) is one of the main limiting nutrients for plant growth and crop yield. It is well documented that changes in nitrate availability, the main N source found in agricultural soils, influences a myriad of developmental programs and processes including the plant defense response. Indeed, many agronomical reports indicate that the plant N nutritional status influences their ability to respond effectively when challenged by different pathogens. However, the molecular mechanisms involved in N-modulation of plant susceptibility to pathogens are poorly characterized. In this work, we show that Solanum lycopersicum defense response to the necrotrophic fungus Botrytis cinerea is affected by plant N availability, with higher susceptibility in nitrate-limiting conditions. Global gene expression responses of tomato against B. cinerea under contrasting nitrate conditions reveals that plant primary metabolism is affected by the fungal infection regardless of N regimes. This result suggests that differential susceptibility to pathogen attack under contrasting N conditions is not only explained by a metabolic alteration. We used a systems biology approach to identify the transcriptional regulatory network implicated in plant response to the fungus infection under contrasting nitrate conditions. Interestingly, hub genes in this network are known key transcription factors involved in ethylene and jasmonic acid signaling. This result positions these hormones as key integrators of nitrate and defense against B. cinerea in tomato plants. Our results provide insights into potential crosstalk mechanisms between necrotrophic defense response and N status in plants. To better understand the molecular changes underlying the impact of nitrate availability on plant susceptibility to B. cinerea, we performed plant transcriptome profiling assays on mock-treated (3 biological replicates) and infected plants ( 3 biological replicates) grown under three N conditions, using GeneChip Tomato Genome Arrays (Affymetrix).