Proteomics

Dataset Information

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Temporal regulation of Lsp1 O-GlcNAcylation and phosphorylation duringactivated Bcellsapoptosis


ABSTRACT: Here, we decipher the molecular mechanisms bridging B cell activation and apoptosis mediated by post-translational modification(PTM). We found that O-GlcNAcase inhibition enhances B cell activation and apoptosisinduced by B cell receptor (BCR)cross-linking. This proteome-scale analysis of the functional interplay between protein O-GlcNAcylation and phosphorylation in stimulated mouse primary B cells identified313O-GlcNAcylation-dependent phosphositeson 224 phosphoproteins.Among these phosphoproteins, temporal regulation ofthe O-GlcNAcylation and phosphorylation of lymphocyte-specific protein-1 (Lsp1) is a key switch that triggersapoptosis in activated B cells. O-GlcNAcylation at S209 of Lsp1, identified as the major O-GlcNAc site on Lsp1 by both electron-transfer dissociationand collision-induced dissociationtandem mass spectrometry,was a prerequisite for the recruitment of its kinase, PKC-β1, to induce S243 phosphorylation, leading to ERK activation and down-regulation of BCL-2 and BCL-xL. Thus, we demonstrate the critical PTM interplay of Lsp1 that transmits signals for initiating apoptosis after BCR ligation.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): B Cell

DISEASE(S): Disease Free

SUBMITTER: Hsin-Yi Wu  

LAB HEAD: Yu-Ju Chen

PROVIDER: PXD003788 | Pride | 2016-09-26

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
B-cell-IPI-mouse_7ppm_p005.dat Other
F022814_IgM10m.dat Other
F022832_Notreat.dat Other
Igm10min-1.RAW Raw
Igm10min-2.RAW Raw
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Publications

Temporal regulation of Lsp1 O-GlcNAcylation and phosphorylation during apoptosis of activated B cells.

Wu Jung-Lin JL   Wu Hsin-Yi HY   Tsai Dong-Yan DY   Chiang Ming-Feng MF   Chen Yi-Ju YJ   Gao Shijay S   Lin Chun-Cheng CC   Lin Chun-Hung CH   Khoo Kay-Hooi KH   Chen Yu-Ju YJ   Lin Kuo-I KI  

Nature communications 20160824


Crosslinking of B-cell receptor (BCR) sets off an apoptosis programme, but the underlying pathways remain obscure. Here we decipher the molecular mechanisms bridging B-cell activation and apoptosis mediated by post-translational modification (PTM). We find that O-GlcNAcase inhibition enhances B-cell activation and apoptosis induced by BCR crosslinking. This proteome-scale analysis of the functional interplay between protein O-GlcNAcylation and phosphorylation in stimulated mouse primary B cells  ...[more]

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