Proteomics

Dataset Information

0

Interactomics of the RLTPR protein explain its essential role for costimulation via CD28

ABSTRACT: The RLTPR cytosolic protein has an essential role in CD28 costimulation but its mode of action and importance in human T cells remain elusive. Here, using mass spectrometry we showed that CD28, RLTPR and the CARMA1 cytosolic adaptor physically associate in mouse T cells. Although RLTPR is thought to function as an actin-uncapping protein, this property was dispensable for CD28 costimulation. Our findings underpin the convergent function of RLTPR in T cells and suggest that its scaffolding role predominates during CD28 costimulation.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): T Cell

SUBMITTER: Luc Camoin  

LAB HEAD: Luc Camoin

PROVIDER: PXD003870 | Pride | 2016-09-27

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
131027_10_Run1.raw Raw
131027_10_Run2.raw Raw
131027_10_Run3.raw Raw
131027_1_Run1.raw Raw
131027_1_Run2.raw Raw
Items per page:
1 - 5 of 137
altmetric image

Publications

The RLTPR cytosolic protein, also known as CARMIL2, is essential for CD28 co-stimulation in mice, but its importance in human T cells and mode of action remain elusive. Here, using affinity purification followed by mass spectrometry analysis, we showed that RLTPR acts as a scaffold, bridging CD28 to the CARD11/CARMA1 cytosolic adaptor and to the NF-κB signaling pathway, and identified proteins not found before within the CD28 signaling pathway. We further demonstrated that RLTPR is essential for  ...[more]

Similar Datasets

Proteomics Treg cells from human blood differentiate into non-lymphoid tissue-resident effector cells upon TNFR2 costimulation
2024-01-24 | PXD048248 | Pride
Transcriptomics CD28-CBCA driven PKM splicing licenses CD8 T cell glycolysis and effector function
2023-09-01 | GSE168824 | GEO
Genomics Effect of costimulation on microRNAs levels in human naïve CD4 T cells
2012-02-25 | GSE36071 | GEO
Transcriptomics Effect of costimulation on microRNAs levels in human naïve CD4 T cells
2012-02-25 | E-GEOD-36071 | biostudies-arrayexpress
Transcriptomics Analysis of Th17 gene signature in the presence of CD28 costimulation in human CD4 naïve T cells
2018-03-12 | GSE110097 | GEO
Transcriptomics Myeloid Niches Sustain Antitumor T Cells and License PD-1 Blockade via CD28 Costimulation
2021-10-12 | GSE178245 | GEO
Proteomics Mus musculus primary CD4+ T cell di-glycine remnant and whole cell proteomics
2019-05-09 | PXD012831 | Pride
Transcriptomics c-Kit signaling potentiates CAR T-cell efficacy in solid tumors by CD28- and IL-2–independent costimulation
2023-04-24 | GSE229026 | GEO
Proteomics Dissecting the antibacterial activity of Oxadiazolone-core derivatives against Mycobacterium abscessus
2020-08-14 | PXD015680 | Pride
Proteomics Cyclipostins and Cyclophostin analogs impair growth of Mycobacterium abscessus by inhibiting enzymes involved in many physiological processes
2019-10-08 | PXD014255 | Pride