Dataset Information

Ability of the marine bacterium Pseudomonas fluorescens BA3SM1 tocounteract the toxicity of CdSe nanoparticles

ABSTRACT: CdSe nanoparticles (CdSe NPs) are extensively used in the industry of renewable energies and it is regrettably expectedthat these pollutants will sometime soon appear in marine environmentthrough surface runoff, urban effluents and rivers. Bacteria living in estuarine and coastal sediments will be among the first targets of these new pollutants. The pseudomonads are frequently encountered in these ecosystems. They are involved in several biogeochemical cycles and are known for their high resistance to pollutants. Consequently, this study focussing on the effect of CdSe NPs on the marine strain P. fluorescensBA3SM1 is highly relevant for a number of reasons. First, it aims at improving knowledge about the interactions between bacteria and NPs. This is fundamental to use effectively NPs against pathogenic bacteria. Secondly, this study shows that CdSe NPs of 8 nm in diameter cause a decrease in the secretion of siderophorepyoverdine, a secondary metabolite having a key role in microbial ecology and also employed as a virulence factor in human pathogenic strains such as P. aeruginosa. Consequently, this study highlights that CdSe NPs can have an impact on secondary metabolism of bacteria with environmental and medical implications.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Pseudomonas Fluorescens Pf5

SUBMITTER: Lauriane Kuhn

LAB HEAD: Isabelle Poirier

PROVIDER: PXD004012 | Pride | 2016-08-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2014_32_I.Poirier_850ng_3-1.wiff	Wiff
	2014_32_I.Poirier_850ng_3-1.wiff.scan	Wiff
	2014_32_I.Poirier_850ng_3-2.wiff	Wiff
	2014_32_I.Poirier_850ng_3-2.wiff.scan	Wiff
	2014_32_I.Poirier_850ng_3-3.wiff	Wiff

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Publications

Ability of the marine bacterium Pseudomonas fluorescens BA3SM1 to counteract the toxicity of CdSe nanoparticles.

Poirier Isabelle I Kuhn Lauriane L Demortière Arnaud A Mirvaux Boris B Hammann Philippe P Chicher Johana J Caplat Christelle C Pallud Marie M Bertrand Martine M

Journal of proteomics 20160811

<h4>Unlabelled</h4>In the marine environment, bacteria from estuarine and coastal sediments are among the first targets of nanoparticle pollution; it is therefore relevant to improve the knowledge of interactions between bacteria and nanoparticles. In this work, the response of the marine bacterium Pseudomonas fluorescens BA3SM1 to CdSe nanocrystals (CdSe NPs) of 3nm (NP3) and 8nm (NP8) in diameter was evaluated through microscopic, physiological, biochemical and proteomic approaches. Transmissi ...[more]

PMID: 27523480

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Project description:Transcriptional profiling of the digestive gland tissue of female mussel Mytilus galloprovincialis exposed to TCDD, n-TiO2 and their binary mixture Background: Exposure of marine organisms to pollutant mixtures may affect the pattern of contaminant uptake/bioaccumulation, as well as of gene expression in the tissues. Despite the growing concern over the potential biological impact of nanoparticles (NPs) in the aquatic environment, little is known about their interactions with other pollutants.We have recently shown that in the marine mussel Mytilus galloprovincialis exposure to n-TiO2, one of the most widespread type of NPs in use, in combination with 2,3,7,8-TCDD, chosen as model organic xenobiotic, can exert antagonistic or synergistic effects on different biomarkers from the molecular to the tissue level, depending on cell/tissue and type of measured response. An integrated approach involving immunhistochemical and transcriptomic analysis was employed to clarify the itteractive effects of n-TiO2 and TCDD in mussels digestive gland. In particular,TCDD bioaccumulation was evaluated utilizing specific anti-TCDD fluorescent antibodies. Moreover, immunohistochemical evaluation of antioxidant and cytoskeletal components was performed. To provide clues about how the molecular response to the investigated compounds is modulated, we used a cDNA microarray with1673 sequences. In animals exposed only to TiO2, functional genomics analysis of the microarray data (48 differentially expressed genes (DEGs)) highlighted three biological processes, largely dominated by the up-regulation of microtubule-based movement-related genes. Exposure to 2,3,7,8-TCDD yielded 49 DEGs exhibiting distinct patterns in terms of biological processes. Finally, exposure to the mixture rendered 62 GEGs characterized by the regulation of response to chemical stimulus, microtubule-based movement and intracellular signal transduction. Our data should be carefully considered in view of the biological effects of emerging pollutants, particularly in case of mixture chemicals. Transcriptional profiling of the digestive gland tissue of female mussel Mytilus galloprovincialis exposed to TCDD, n-TiO2 and their binary mixture