Comprehensive functional analysis of Escherichia coli ribosomal RNA methyltransferases
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ABSTRACT: We analyzed total proteome of three methyltransferase gene knockouts strains of Escherichia coli: ΔrsmF (JW5301), ΔrlmC (JW2756), ΔrlmE (JW3146) from Keio collection and wild type (WT). Proteins were assessed using IDA approach (i.e. Information Dependent Acquisition) and SWATH (Data-Independent Acquisition) on Sciex TripleTof 5600+ mass‐spectrometer with a NanoSpray III ion source (ABSciex, Canada) coupled to a NanoLC Ultra 2D+ nano‐HPLC system (Eksigent). Dataset covers 16 samples.
INSTRUMENT(S): TripleTOF 5600
ORGANISM(S): Escherichia Coli
SUBMITTER: Olga Bukato
LAB HEAD: Petr Sergiev
PROVIDER: PXD017171 | Pride | 2020-01-21
REPOSITORIES: Pride
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