Dataset Information

Dynamics of the mouse brain cortical synaptic proteome during postnatal brain development

ABSTRACT: Development of the brain involves the formation and maturation of numerous synapses. This process requires prominent changes of the synaptic proteome and potentially involves thousands of different proteins at every synapse. To date the proteome analysis of synapse development has been studied sparsely. Here, we analyzed the cortical synaptic membrane proteome of juvenile [postnatal day 9 (P9), P15, P21, P27], adolescent (P35) and different adult ages (P70, P140, P280) of C57Bl6/J mice. Using a quantitative proteomics workflow we quantified 1560 proteins of which 696 showed statistically significant differences over time. Proteins of the presynaptic active-zone and postsynaptic element showed increased levels over time; proteins involved in protein synthesis or neurite outgrowth generally decreased in abundance. In several cases, proteins from a single functional molecular entity, e.g., subunits of the NMDA receptor, showed profound differences in their temporal regulation, which may reflect specific synaptic development features of connectivity, strength and plasticity.

INSTRUMENT(S): 4800 Proteomics Analyzer

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Pim van Nierop

LAB HEAD: Ka Wan Li

PROVIDER: PXD004129 | Pride | 2018-10-26

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	SupplementalTable10_Experiment3_ProteinSummary.txt	Txt
	SupplementalTable1_Combined-search_PeptideSummary.txt	Txt
	SupplementalTable2_Combined-search_ProteinSummary.txt	Txt
	SupplementalTable5_Experiment1_PeptideSummary.txt	Txt
	SupplementalTable6_Experiment1_ProteinSummary.txt	Txt

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Publications

Dynamics of the mouse brain cortical synaptic proteome during postnatal brain development.

Gonzalez-Lozano Miguel A MA Klemmer Patricia P Gebuis Titia T Hassan Chopie C van Nierop Pim P van Kesteren Ronald E RE Smit August B AB Li Ka Wan KW

Scientific reports 20161017

Development of the brain involves the formation and maturation of numerous synapses. This process requires prominent changes of the synaptic proteome and potentially involves thousands of different proteins at every synapse. To date the proteome analysis of synapse development has been studied sparsely. Here, we analyzed the cortical synaptic membrane proteome of juvenile postnatal days 9 (P9), P15, P21, P27, adolescent (P35) and different adult ages P70, P140 and P280 of C57Bl6/J mice. Using a ...[more]

PMID: 27748445

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