Dataset Information

Uterine flushing proteome of tammar wallaby from diapause to reactivation and late gestation

ABSTRACT: The tammar blastocyst remains in embryonic diapause until it receives a signal from the uterine endometrium via its secretions.This study analaysed uterine flushings from diapause, different stages of reactivation upto late gestation.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Macropus Eugenii

DISEASE(S): Disease Free

SUBMITTER: Florine Cynthia Martin

LAB HEAD: Geoff Shaw

PROVIDER: PXD004170 | Pride | 2016-08-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	140612_cynthia_4940_gr.zip	Other
	140612_cynthia_4940_ngr.zip	Other
	140612_cynthia_5235_gr.zip	Other
	140612_cynthia_5235_ngr.zip	Other
	140612_cynthia_5511_gr.zip	Other

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Project description:The endometrium contains a distinct population of immune cells consisting of 70% natural killer (NK) cells that undergo cyclic changes during the menstrual cycle. However, how these uterine NK (uNK) cells interact with uterine stromal cells (SC) remains unclear. We therefore investigated the paracrine effect of medium conditioned by uNK cells on the gene expression profile of endometrial SC in-vitro using a cDNA Microarray. Our results, verified by real-time PCR and ELISA, reveal that soluble factors from uNK cells substantially alter endometrial SC gene expression. The largest group of up-regulated genes found were chemokines and cytokines, including IL-15 and IL-15RÎ±. The latter could produce a niche for uNK cells allowing proliferation within and recruitment into the uterus, as seen in bone marrow. In addition, the most abundantly up-regulated genes, including IL-8, CCL8 and CXCL1 have also been shown to be stimulated by contact of SC with trophoblast, suggesting that uNK cells work synergistically to support the initial trophoblast migration during implantation. Overall this study demonstrates for the first time the paracrine communication between uNK cells and uterine SC, and adds to the understanding of how the uterine immune system contributes to the changes seen within the cycling endometrium. Keywords: Response of endometrial stromal cells to uNK conditioned medium This study was designed to identify the response of non-decidualised stromal cells from the endometrium, to soluble factors secreted by uterine NK cells. Endometrial stromal cells were isolated from 7 patients and treated with control medium or medium conditioned by uterine Nk cells. THE 'REF' COLUMN ON EACH ARRAY IS THE SIGNAL PRODUCED BY A COMMON REFERNCE RNA SAMPLE THAT WAS LABELLED AS A SINGLE BATCH SAMPLE AND HYBRIDISED TO ALL THE ARRAYS- IT IS A 'COMMON REFERENCE'. THE 'TEST' SAMPLE COMPRISES EACH INDIVIDUAL SAMPLE OF CELLS TREATED AS DESCRIBED IN THE SERIES SUBMISSION. FOR EXAMPLE; SAMPLE Y1 (gsm2435820) IS RNA FROM PATIENT 1 TREATED WITH CONTROL MEDIUM, SAMPLE G1 (GSM245371) IS RNA FROM PATIENT 1 TREATED WITH NK CONDITIONED MEDIUM THESE TWO SAMPLES THEREFORE FORM A PAIR- IE CELLS FROM THE SAME PATIENT TREATED WITH CONTROL OR NK-CONDITIONED MIDIUM. Y2,G2 ARE FROM PATIENT 2, ETC

Dataset Information

Uterine flushing proteome of tammar wallaby from diapause to reactivation and late gestation

Dataset's files

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