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Plasma Biomarker Profile Altered Through Variable Blood Storage

ABSTRACT: A major confounding factor for defining disease-specific molecular signatures is the variation in handling and processing of clinical samples. To address this, we assessed how the plasma proteome and degradome changes when whole blood remains at ambient temperature (AT) following collection and prior to processing.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Philip Charles

LAB HEAD: Benedikt Kessler

PROVIDER: PXD004205 | Pride | 2018-10-26

REPOSITORIES: Pride

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			Action	DRS
	F159439.mzid.gz	Mzid
	F159439.pride.mztab.gz	Mztab
	OTE0346_MariaK_Deg1_0.raw	Raw
	OTE0346_MariaK_Deg1_1.raw	Raw
	OTE0346_MariaK_Deg1_2.raw	Raw

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Publications

Plasma degradome affected by variable storage of human blood.

Kaisar Maria M van Dullemen Leon F A LFA Thézénas Marie-Laëtitia ML Zeeshan Akhtar M M Huang Honglei H Rendel Sandrine S Charles Philip D PD Fischer Roman R Ploeg Rutger J RJ Kessler Benedikt M BM

Clinical proteomics 20160926

<h4>Background</h4>The successful application of-omics technologies in the discovery of novel biomarkers and targets of therapeutic interventions is facilitated by large collections of well curated clinical samples stored in bio banks. Mining the plasma proteome holds promise to improve our understanding of disease mechanisms and may represent a source of biomarkers. However, a major confounding factor for defining disease-specific proteomic signatures in plasma is the variation in handling and ...[more]

PMID: 27708557

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Project description:For around ten years, microarrays have been suggested for the diagnosis of ionizing radiation exposure. We assessed for the first time the relevance of gene expression profiling in a real accidental case. This study was performed on peripheral blood mononuclear cells of 41 potential victims. The different strategies of analysis highlighted a huge effect of the blood sample handling on the gene expression profiles. This effect was so high that it could mask specific modulations as a potential effect of ionizing radiation exposure. Thus, we assessed a new way of blood sampling adapted to gene expression analysis: PAXgene. With this method, more than 70% of the modulations of gene expression induced 3 hours after an ex vivo exposure to 0.5 Gy were preserved even in a 24-hour delayed analysis (as for transportation of blood sample from the accident location to the laboratory). We validated a new methodology in order to propose a new strategy of blood sampling and handling for gene profiling. This system could be used in case of accidental overexposure to study whether gene expression is a relevant biomarker of ionizing radiation exposure. Radiation induced gene expression in human blood was measured at 3 hours after exposure to doses of 0 and 0.5 grays. Following the incubation of 3 hours at 37Â°C, the RNA extractions were performed either immediately or 24h later (as for transportation of blood sample at room temperature). Two different blood preservation methods were compared: classical anticoagulant and PAXgene Blood RNA System. Venous blood samples of 6 donors were used (3 for anticoagulant study, 3 for Paxgene study). Each sample was hybridized twice.

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