Proteomics

Dataset Information

0

Comparison of performances of automatic versus manual liquid handling in density-based separation of extracellular vesicles from plasma and urine


ABSTRACT: Extracellular vesicles (EV) in body fluids are extensively studied as potential biomarkers for numerous diseases. Major impediments of EV-based biomarker discovery include the manual labor, and the specificity and reproducibility of EV sample preparation. To tackle this, we present an automated liquid handling workstation for the density-based separation of EV from human body fluids and compare its performance to manual handling by (in)experienced researchers. To validate automated density-based separation of EVs from human body fluids, including blood plasma and urine, we assess variation, yield and purity by mass spectrometry-based proteomics.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine, Blood Plasma

SUBMITTER: Pekka Rappu  

LAB HEAD: Olivier De Wever

PROVIDER: PXD032394 | Pride | 2023-05-10

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
200207_UH1G_1.raw Raw
200207_UH1G_2.raw Raw
200207_UH1G_3.raw Raw
200207_UH2_1.raw Raw
200207_UH2_2.raw Raw
Items per page:
1 - 5 of 222

Similar Datasets

2021-09-10 | E-MTAB-10447 | biostudies-arrayexpress
2016-08-20 | E-GEOD-85830 | biostudies-arrayexpress
2022-11-16 | E-MTAB-11910 | biostudies-arrayexpress
2020-02-17 | PXD017273 | Pride
2021-12-28 | E-MTAB-11020 | biostudies-arrayexpress
2022-10-20 | PXD033867 | Pride
2017-10-06 | E-MTAB-5164 | biostudies-arrayexpress
2022-02-15 | PXD020584 | Pride
2017-10-06 | E-MTAB-5165 | biostudies-arrayexpress
2013-06-19 | E-GEOD-47897 | biostudies-arrayexpress