Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Homo Sapiens (human) Aeropyrum Pernix
TISSUE(S): Cell Suspension Culture, Cell Culture
SUBMITTER: Marko Fonovic
LAB HEAD: prof. dr. Marko Fonovic
PROVIDER: PXD004218 | Pride | 2017-07-06
REPOSITORIES: Pride
The EMBO journal 20170721 16
Determination of protease specificity is of crucial importance for understanding protease function. We have developed the first gel-based label-free proteomic approach (DIPPS-direct in-gel profiling of protease specificity) that enables quick and reliable determination of protease cleavage specificities under large variety of experimental conditions. The methodology is based on in-gel digestion of the gel-separated proteome with the studied protease, enrichment of cleaved peptides by gel extract ...[more]