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Myosin VIIA 138-1 immunoaffinity purification

ABSTRACT: Through its activity in stereocilia, MYO7A (myosin VIIA) is essential for hair cell function in the inner ear. Utilizing multiple stages of immunoaffinity enrichment, we have developed a strategy that allows us to partially purify stereocilia membranes from thousands of chick inner ears and isolate low-abundance MYO7A protein complexes from those membranes. By analysis of MYO7A and co-purifying molecules with shotgun and targeted mass spectrometry, we demonstrated that MYO7A forms a complex with PDZD7, a paralog of USH1C and DFNB31, which has been shown to localize to the ankle-link region of stereocilia.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Ear Hair Cell, Inner Ear

SUBMITTER: Peter Barr-Gillespie

LAB HEAD: Peter Barr-Gillespie

PROVIDER: PXD004221 | Pride | 2016-08-16

REPOSITORIES: Pride

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			Action	DRS
	GIL1467_ControlMagIP_Directinjection_15ul_HCDonly_25cm_150min_20140307_OT.raw	Raw
	GIL1467_MYO7a_MagIP_Directinjection_15ul_HCDonly_25cm_150min_20140307_OT.raw	Raw
	GIL1649_IP_CTRL_2_shotgun_20150324_OT.raw	Raw
	GIL1649_IP_CTRL_shotgun_20150216_OT.raw	Raw
	GIL1649_IP_CTRL_shotgun_20150223_OT.raw	Raw

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