Dataset Information

Site-specific glycosylation of donkey milk lactoferrin investigated by High Resolution Mass Spectrometry

ABSTRACT: A comprehensive glycosylation profile of donkey lactoferrin, isolated by ion exchange chromatography from an individual milk sample, was obtained by means of chymotryptic digestion, TiO2 and HILIC enrichment, reversed-phase high performance liquid chromatography, electrospray mass spectrometry, and high collision dissociation fragmentation. The results obtained allowed the identification of 26 different glycan structures, including high mannose, complex and hybrid N-glycans, linked to the protein backbone via an amide bond to asparagine residues located at the positions 137, 281 and 476. Altogether, the N-glycan structures determined revealed that in donkey milk lactoferrin most of the N-glycans identified are neutral complex/hybrid. Actually, 10 neutral non-fucosylated complex/hybrid N-glycans and 4 neutral fucosylated complex/hybrid N-glycans were found. In addition, 2 high mannose N-glycans, 4 sialylated fucosylated complex/hybrid N-glycans and 6 sialylated non-fucosylatedN-glycans, one of which containing N-glycolylneuramin acid (Neu5Gc), were found. A comparison of the glycosylation profile of donkey milk lactoferrin with respect to that of human, bovine and goat milk lactoferrin is reported.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Equus Asinus (donkey)

TISSUE(S): Milk

SUBMITTER: Salvatore Foti

LAB HEAD: Salvatore Foti

PROVIDER: PXD004289 | Pride | 2016-08-25

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Qex02580-chimotrypsin-TiO2.mgf	Mgf
	Qex02580-chimotrypsin-TiO2.raw	Raw
	Qex02581-chimotrypsin-TiO2.raw	Raw
	Qex02581-chimotrypsin-Tio2.mgf	Mgf
	Qex02586-chimotrypsin-TiO2-HILIC.mgf	Mgf

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Publications

Site-specific glycosylation of donkey milk lactoferrin investigated by high-resolution mass spectrometry.

Gallina Serafina S Saletti Rosaria R Cunsolo Vincenzo V Muccilli Vera V Foti Salvatore S Roepstorff Peter P Rasmussen Morten I MI

Amino acids 20160822 12

A comprehensive monosaccharide composition of the N-glycans of donkey milk lactoferrin, isolated by ion exchange chromatography from an individual milk sample, was obtained by means of chymotryptic digestion, TiO<sub>2</sub> and HILIC enrichment, reversed-phase high-performance liquid chromatography, electrospray mass spectrometry, and high collision dissociation fragmentation. The results obtained allowed identifying 26 different glycan structures, including high mannose, complex and hybrid N-g ...[more]

PMID: 27550041

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