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Glycoproteomic analysis of Fut8 knock out/wild type mouse brain, Human IgG and Human Serum.

ABSTRACT: In this study, we provide a simple and straightforward method to distinguish core-fucosylated glycans from antenna-fucosylated glycans by systematically evaluating the extent of fucose migration based on the feature Y ions by using Fut8 knockout (Fut8−/−) mouse brain under HCD energy of 20%. The method was further evaluated by multiple different approaches including validation by standard glycopeptides, evaluation in silico, comparison under the lower energy (15%), and applied to analyze core-fucosylated glycans in wide-type (Fut8+/+) mouse brain.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Brain, Blood Serum

SUBMITTER: Zexuan Chen

LAB HEAD: Shisheng Sun

PROVIDER: PXD035158 | Pride | 2022-11-14

REPOSITORIES: Pride

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			Action	DRS
	Fut8_KO_max_IGP_mousebrain_1.raw	Raw
	Fut8_KO_max_IGP_mousebrain_1_result.xlsx	Xlsx
	Fut8_KO_max_IGP_mousebrain_2.raw	Raw
	Fut8_KO_max_IGP_mousebrain_2_result.xlsx	Xlsx
	Fut8_KO_max_IGP_mousebrain_3.raw	Raw

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