Dataset Information

Quantitative proteomics of liver from methylmercury-exposed Atlantic cod

ABSTRACT: In order to investigate the underlying mechanisms of methylmecury (MeHg)-mediated toxicity to Atlantic cod (Gadus morhua), we analyzed the liver proteome of fish exposed in vivo to MeHg (0, 0.5, 2 mg/kg body weight) for 2 weeks. Label-free quantitative mass spectrometry enabled quantification of 1143 proteins, and 125 were differentially regulated between MeHg-treated samples and controls. Six proteins among the top differentially regulated (T23O, GLNA EPS8L2, APOA4, RAP1B, CZTZ) were analyzed using selected reaction monitoring (SRM). Supported by bioinformatics analyses, we conclude that MeHg disrupts mainly redox homeostasis and energy generating metabolic pathways in cod liver, the latter potentially modulated through MeHg-induced oxidative stress.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Gadus Morhua (atlantic Cod)

TISSUE(S): Liver

SUBMITTER: Odd Andre Karlsen

LAB HEAD: Odd Andre Karlsen

PROVIDER: PXD004311 | Pride | 2016-08-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Gadus_morhua_gadMor1_75_pep_all_generic.fasta_concatenated_target_decoy.fasta	Fasta
	MeHg_Ensemble_MSGF_Xt_Amanda_PeptideShaker_Export.txt	Txt
	MeHg_Ensemble_MSGF_Xt_Amanda_Progenesis_export_Peptides.csv	Csv
	MeHg_Ensemble_MSGF_Xt_Amanda_Progenesis_export_Proteins.xlsx	Xlsx
	OAK_05_1.raw	Raw

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Publications

Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism.

Yadetie Fekadu F Bjørneklett Silje S Garberg Hilde Kristin HK Oveland Eystein E Berven Frode F Goksøyr Anders A Karlsen Odd André OA

BMC genomics 20160805

<h4>Background</h4>Methylmecury (MeHg) is a widely distributed environmental pollutant with considerable risk to both human health and wildlife. To gain better insight into the underlying mechanisms of MeHg-mediated toxicity, we have used label-free quantitative mass spectrometry to analyze the liver proteome of Atlantic cod (Gadus morhua) exposed in vivo to MeHg (0, 0.5, 2 mg/kg body weight) for 2 weeks.<h4>Results</h4>Out of a toltal of 1143 proteins quantified, 125 proteins were differentiall ...[more]

PMID: 27496535

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