Dataset Information

HeLa proteome of 12,250 protein-coding genes

ABSTRACT: We developed an optimized multi-shot proteomics workflow based on high-resolution offline high pH reversed-phase peptide separation of high peptide loads collecting many fractions that were in turn analyzed by short online chromatographic separations and fast peptide sequencing using orbitrap tandem mass spectrometry.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Liver, Colon

SUBMITTER: Christian Kelstrup

LAB HEAD: Jesper V. Olsen

PROVIDER: PXD004452 | Pride | 2017-06-12

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20150115_QE3_UPLC9_DBJ_SA_Waters_70frac_no_concatenation_rep1_1.raw	Raw
	20150115_QE3_UPLC9_DBJ_SA_Waters_70frac_no_concatenation_rep1_10.raw	Raw
	20150115_QE3_UPLC9_DBJ_SA_Waters_70frac_no_concatenation_rep1_11.raw	Raw
	20150115_QE3_UPLC9_DBJ_SA_Waters_70frac_no_concatenation_rep1_12.raw	Raw
	20150115_QE3_UPLC9_DBJ_SA_Waters_70frac_no_concatenation_rep1_13.raw	Raw

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Publications

An Optimized Shotgun Strategy for the Rapid Generation of Comprehensive Human Proteomes.

Bekker-Jensen Dorte B DB Kelstrup Christian D CD Batth Tanveer S TS Larsen Sara C SC Haldrup Christa C Bramsen Jesper B JB Sørensen Karina D KD Høyer Søren S Ørntoft Torben F TF Andersen Claus L CL Nielsen Michael L ML Olsen Jesper V JV

Cell systems 20170607 6

This study investigates the challenge of comprehensively cataloging the complete human proteome from a single-cell type using mass spectrometry (MS)-based shotgun proteomics. We modify a classical two-dimensional high-resolution reversed-phase peptide fractionation scheme and optimize a protocol that provides sufficient peak capacity to saturate the sequencing speed of modern MS instruments. This strategy enables the deepest proteome of a human single-cell type to date, with the HeLa proteome se ...[more]

PMID: 28601559

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