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Proteomic analysis of T cell differentiation

ABSTRACT: T helper cells (Th) play an important role guarding, regulating and modulating immune responses. The different Th lineages fulfill different tasks in response to infections. The two best defined subtypes are Th1 (involved in cellular immunity) and Th2 (humoral immunity). The cytokine environment after activation determines the differentiation path of a Th cell. We defined a strategy to investigate the differentiation signature of T helper cells with a proteomics approach. Murine primary naïve CD4+ T-cells from spleen were differentiated and activated in vitro. After 3 days of differentiation proteins were analysed. The proteomic profile of the Th1 and Th2 cells will help understand these phenotypes better, which is important in finding therapeutic targets for disease and for the development of effective vaccines.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spleen, T Cell

SUBMITTER: N. Binai

LAB HEAD: Albert J.R. Heck

PROVIDER: PXD004532 | Pride | 2019-02-14

REPOSITORIES: Pride

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			Action	DRS
	OR7_20140729_NB_Virgo_Mix15_F10.raw	Raw
	OR7_20140729_NB_Virgo_Mix15_F10_2.raw	Raw
	OR7_20140729_NB_Virgo_Mix15_F11.raw	Raw
	OR7_20140729_NB_Virgo_Mix15_F12.raw	Raw
	OR7_20140729_NB_Virgo_Mix15_F13_17.raw	Raw

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