Proteomics

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Quantitative metaproteomics highlight the metabolic contributions of uncultured phylotypes in a thermophilic anaerobic digester


ABSTRACT: In this study, we used multiple meta-omic approaches to characterize the microbial community and the active metabolic pathways of a stable industrial biogas reactor operating at thermophilic temperatures (60°C) and elevated levels of free ammonia (367 mg NH3-N/L).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Coprothermobacter Methanosaeta

SUBMITTER: Magnus Arntzen  

LAB HEAD: Vincent Eijsink

PROVIDER: PXD004538 | Pride | 2017-06-30

REPOSITORIES: Pride

Dataset's files

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Action DRS
160304_Exp66_FREVAR_1_1.raw Raw
160304_Exp66_FREVAR_1_10.raw Raw
160304_Exp66_FREVAR_1_11.raw Raw
160304_Exp66_FREVAR_1_12.raw Raw
160304_Exp66_FREVAR_1_13.raw Raw
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Publications

Quantitative Metaproteomics Highlight the Metabolic Contributions of Uncultured Phylotypes in a Thermophilic Anaerobic Digester.

Hagen Live H LH   Frank Jeremy A JA   Zamanzadeh Mirzaman M   Eijsink Vincent G H VGH   Pope Phillip B PB   Horn Svein J SJ   Arntzen Magnus Ø MØ  

Applied and environmental microbiology 20161230 2


In this study, we used multiple meta-omic approaches to characterize the microbial community and the active metabolic pathways of a stable industrial biogas reactor with food waste as the dominant feedstock, operating at thermophilic temperatures (60°C) and elevated levels of free ammonia (367 mg/liter NH<sub>3</sub>-N). The microbial community was strongly dominated (76% of all 16S rRNA amplicon sequences) by populations closely related to the proteolytic bacterium Coprothermobacter proteolytic  ...[more]

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