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LC-MS/MS of dithiothreitol-extracted proteins from the insect fungal pathogen Beauveria bassiana hyphal bodies (in vivo blastospores)

ABSTRACT: Proteins were extracted using 2 mM DTT from Beauveria bassiana hyphal bodies (in vivo blastospores) were identified and quantified using Label-free quantitative mass spectrometry.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Beauveria Bassiana Cgmcc 7.34

TISSUE(S): Blastopore, Fungal Asexual Spore

SUBMITTER: zhi Yang

LAB HEAD: Yongjun Zhang

PROVIDER: PXD004544 | Pride | 2017-01-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2-HB.group	Other
	HB.group	Other
	beauveriabassiana-HB-1-2.mgf	Mgf
	beauveriabassiana-HB-1-2.wiff	Wiff
	beauveriabassiana-HB-1.mgf	Mgf

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1 - 5 of 26

Publications

Correlation of cell surface proteins of distinct Beauveria bassiana cell types and adaption to varied environment and interaction with the host insect.

Yang Zhi Z Jiang Hongyan H Zhao Xin X Lu Zhuoyue Z Luo Zhibing Z Li Xuebing X Zhao Jing J Zhang Yongjun Y

Fungal genetics and biology : FG & B 20161228

The insect fungal pathogen Beauveria bassiana produces a number of distinct cell types that include aerial conidia, blastospores and haemolymph-derived cells, termed hyphal bodies, to adapt varied environment niches and within the host insect. These cells display distinct biochemical properties and surface structures, and a highly ordered outermost brush-like structure uniquely present on hyphal bodies, but not on any in vitro cells. Here, we found that the outermost structure on the hyphal bodi ...[more]

PMID: 28040530

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LC-MS/MS of dithiothreitol-extracted proteins from the insect fungal pathogen Beauveria bassiana blastospores

Project description:Proteins were extracted using 2 mM DTT from Beauveria bassiana blastospores were identified and quantified using Label-free quantitative mass spectrometry.

2017-01-04 | PXD004535 | Pride

LC-MS/MS of dithiothreitol-extracted proteins from the insect fungal pathogen Beauveria bassiana conidia

Project description:Proteins were extracted using 2 mM DTT from Beauveria bassiana conidia were identified and quantified using Label-free quantitative mass spectrometry.

2022-03-02 | PXD004474 | Pride

LC-MS/MS of dithiothreitol-extracted proteins from the insect fungal pathogen Beauveria bassiana conidia

Project description:Proteins were extracted using 2 mM DTT from Beauveria bassiana conidia were identified and quantified using Label-free quantitative mass spectrometry.

2017-01-04 | PXD004470 | Pride

Pancreatic cancer cells treated with anlotinib

Project description:Aberrant regulation of angiogenesis involves in the growth and metastasis of tumors, but angiogenesis inhibitors fail to improve overall survival of pancreatic cancer patients in previous phase III clinical trials. A comprehensive knowledge of the mechanism of angiogenesis inhibitors against pancreatic cancer is helpful for clinical purpose and for the selection of patients who might benefit from the inhibitors. In this work, multi-omics analyses (transcriptomics, proteomics and phosphoproteomics profiling) were carried to delineate the mechanism of anlotinib, a novel angiogenesis inhibitor, against pancreatic cancer cells.

2021-09-09 | PXD023344 | Pride

A proteomics of gills approach to understanding salinity adaptation of Scylla paramamosain

Project description:Scylla paramamosain (Crustacea) is a commercially important euryhaline species distributed along the coast of southern China and other Indo-Pacific countries. However, a sudden variation in salinity will cause injury or even death of S. paramamosain. In this paper, we simulated a sudden decrease in salinity due to heavy precipitation in crab ponds. Comparison of gill microstructures of individuals in the control group and decreased salinity group showed gills became shorter and thicker, while the top of the filaments became swollen and then returned to normal after 120 h. A total of 3962 proteins were identified by proteomic sequencing of gills after 120 h under conditions of decreased salinity. 845 proteins were differentially expressed proteins: 371 up-regulated and 474 down-regulated. Of the enriched KEGG pathways, 20 were up-regulated and 14 were down-regulation (p<0.05). Among the significantly enriched up-regulated pathways, six were associated with amino acid metabolism and three were associated with Na+-K+-ATPase enzymatic activities. Pathways associated with redox metabolism and energy metabolism were identified. These results showed that in response to a decrease in salinity, S. paramamosain could adapt to the environment after 120 h. Molecular mechanism of this adaptation involved amino acid metabolism and Na+-K+-ATPase ion transport. Meanwhile, energy metabolism and redox metabolism were critical to the adaptation to a sudden decrease in salinity. This study, for the first time at the protein level, revealed the molecular mechanisms underlying salinity adaption of S. paramamosain and provides theoretical guidelines for the cultivation of S. paramamosain and other marine crustaceans.

2018-07-31 | PXD009179 | Pride

Dissecting the secretory pathway with electrophoresis reveals intra-Golgi trafficking pathways and enables compilation of sub-Golgi and sub-ER proteomes.

Project description:The Golgi is the hub of the eukaryotic secretory pathway, trafficking proteins and lipids, as well as synthesizing complex sugars. Different biosynthetic reactions are associated with different compartments of its complex architecture. Although pre- and post-Golgi trafficking has been much studied, comparatively little is known about intra-Golgi organization. In this study, secretory vesicles and organelles were separated along an electrophoretic gradient at sub-Golgi resolution, presenting snapshots of the changing relative abundance of hundreds of resident and cargo proteins and glycans in transit through the ER, Golgi compartments and post-Golgi compartments. Furthermore, grouped features in migration profiles reveal the dominant intra-Golgi protein trafficking pathways, showing separate routes for cargo and different groups of resident proteins. As few structural characteristics of proteins or sequence motifs have been associated with specific regions of the Golgi stack, we also carried out a comparative analysis of the transmembrane regions of resident proteins associated with the main migratory profiles identifying the presence of charge amino acids adjacent to the transmembrane helix, exoplasmic Ser and Thr content and helix composition as likely contributors to protein sorting mechanisms.

2019-09-11 | PXD004596 | Pride

Characterization of degradative pathway of diclofenac in Raoultella sp. KDF8 resolved by comparative proteomics and metabolomics study

Project description:The widespread appearance of diclofenac as an emerging contaminant in the environment and its potential impact on living organisms is a matter of concern. Our current work uses proteomics and metabolomics to gain a better understanding of diclofenac biodegradation by the bacterial strain Raoultella sp. KDF8.

2019-05-06 | PXD010643 | Pride

Identification of potential interactors of NKS1 and QUA1

Project description:Adjacent plant cells are connected by specialized cell wall regions, called middle lamellae, which influence critical agricultural characteristics, including fruit ripening and organ abscission. Middle lamellae are enriched in pectin polysaccharides, specifically homogalacturonan (HG). Pectins are made in the Golgi apparatus by the coordinated action of transporters and enzymes. Here, in this project, we identify a plant-specific Arabidopsis DUF1068 protein, called NKS1/ELMO4, that is required for middle lamellae integrity and cell adhesion. We did an immunoprecipitation-mass spectrometry (IP-MS) analysis to identify potential interactors of NKS1. And we also performed an IP-MS analysis of QUA1, which is one of the interactors of NKS1.

2024-03-20 | PXD047727 | Pride

Targeted AURKA degradation: Towards new therapeutic agents for neuroblastoma

Project description:Aurora kinase A (AURKA) is a well-established target in neuroblastoma (NB) due to both its catalytic functions during mitosis and its kinase-independent functions, including stabilization of the key oncoprotein MYCN. We present a structure-activity relationship (SAR) study of MK-5108-derived PROTACs against AURKA by exploring different linker lengths and exit vectors on the thalidomide moiety. PROTAC SK2188 induces the most potent AURKA degradation (DC50,24h 3.9 nM, Dmax,24h 89%) and shows an excellent binding and degradation selectivity profile. Treatment of NGP neuroblastoma cells with SK2188 induced concomitant MYCN degradation, high replication stress/DNA damage levels and apoptosis. Moreover, SK2188 significantly outperforms the parent inhibitor MK-5108 in a cell proliferation screen and patient-derived organoids. Furthermore, altering the attachment point of the PEG linker to the 5-position of thalidomide allowed us to identify a potent AURKA degrader with a linker as short as 2 PEG units. With this, our SAR-study provides interesting lead structures for further optimization and validation of AURKA degradation as a potential therapeutic strategy in neuroblastoma

2023-02-27 | PXD040391 | Pride

metaproteomic analysis of microbiota and metabolic pathways involved in taste formation during Chinese traditional fish sauce

Project description:Complex microbial metabolism is key to taste formation in high-quality fish sauce during fermentation. To guide quality supervising and targeted regulation, we analyzed the function of microbial flora during fermentation based on a previous metagenomic database. Most of the identified genes involved in metabolic functions showed an upward trend in abundance during fermentation. In total, 571 proteins extracted from fish sauce at different fermentation stages were identified. The proteins were mainly derived from Halanaerobium, Psychrobacter, Photobacterium, and Tetragenococcus. Functional annotation showed 15 pathways related to amino acid metabolism, including alanine, aspartate, glutamate, and histidine metabolism; lysine degradation; and arginine biosynthesis.

2022-05-20 | PXD031089 | Pride

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