Dataset Information

Tear proteome analysis by LC-MS/MS

ABSTRACT: We have analysed the tear proteome from multiple volunteers without ocular disease using untargeted LC-MS/MS with and without pre-fractionation of peptide digests. Standard LC-MS/MS was compared to results from fractionation by mixed-mode cation exchange SPE and reverse-phase HPLC fractionation. The combined analysis of all these samples resulted in the identification of 886 proteins.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Lekha Sleno

LAB HEAD: Lekha Sleno

PROVIDER: PXD041752 | Pride | 2023-09-01

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	200701_Tears_Incubation_0min_A2B1_DTT_IAM_A_NewLong60min.wiff	Wiff
	200701_Tears_Incubation_0min_A2B1_DTT_IAM_A_NewLong60min.wiff.scan	Wiff
	200701_Tears_Incubation_0min_A2B1_DTT_IAM_B_NewLong60min.wiff	Wiff
	200701_Tears_Incubation_0min_A2B1_DTT_IAM_B_NewLong60min.wiff.scan	Wiff
	200701_Tears_Incubation_30min_A1B2_DTT_IAM_A_NewLong60min.wiff	Wiff

Items per page:

1 - 5 of 218

Similar Datasets

Project description:Rodents are commonly housed below thermoneutrality (~20°C) 1. Under these conditions there is a substantial effect on rodent physiology including the hyperactivation of brown (BAT) and beige adipose tissue 2. Here, we raised animals from weaning, on an obesogenic diet at thermoneutrality (28°C) to closer mimic human physiology and determine the impact of a) moderate cold exposure (i.e. 20°C, a temperature reduction of ~8°C) or b) treatment with YM-178, a highly-selective, clinically used β3-adrenoreceptor agonist on classical BAT or subcutaneous inguinal (IWAT) beige depots. Under these conditions, uncoupling protein 1 mRNA was undetectable in IWAT in all groups. Maintenance at 20°C drove weight gain and a 125% increase in subcutaneous fat, an effect not seen with YM-178 administration thus suggesting a direct effect of ambient temperature in promoting weight gain and adiposity in obese rats. Using exploratory adipose tissue proteomics we reveal novel processes and pathways associated with cold-induced weight gain in BAT (i.e. histone deacetylation and glycosphingolipid biosynthesis) and IWAT (i.e. NAD+ binding and retinol metabolism). Conversely, YM-178 had minimal metabolic-related effects on BAT and drove a pro-inflammatory phenotype in IWAT. Exercise training elicits diverse effects on brown (BAT) and white adipose tissue (WAT) physiology in rodents housed below their thermoneutral zone (i.e. 28-32°C). In these conditions, BAT is chronically hyperactive and, unlike human residence, closer to thermoneutrality. Therefore, we set out to determine the effects of exercise training in obese animals at 28°C (i.e. thermoneutrality) on BAT and WAT in its basal (i.e. inactive) state. Sprague-Dawley rats (n=12) were housed at thermoneutrality from 3 weeks of age and fed a high-fat diet. At 12 weeks of age half these animals were randomised to 4-weeks of swim-training (1 hour/day, 5 days per week). Following a metabolic assessment interscapular and perivascular BAT and inguinal (I)WAT were taken for analysis of thermogenic genes and the proteome. Exercise attenuated weight gain but did not affect total fat mass or thermogenic gene expression. Proteomics revealed an impact of exercise training on2-oxoglutarate metabolic process, mitochondrial respiratory chain complex IV, carbon metabolism and oxidative phosphorylation. This was accompanied by an upregulation of multiple proteins involved in skeletal muscle physiology suggesting an adipocyte to myocyte switch in BAT. UCP1 mRNA was undetectable in IWAT with proteomics highlighting changes to DNA binding, the positive regulation of apoptosis, HIF-1 signalling and cytokine-cytokine receptor interaction.

Dataset Information

Tear proteome analysis by LC-MS/MS

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets