Proteomics

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HCOV-NL63 spike protein glycopeptides

ABSTRACT: HCoV-NL63 is a coronavirus that can cause severe lower respiratory tract infections requiring hospitalization. Of great interest is understanding the HCoV-NL63 coronavirus spike glycoprotein trimer, which is the conformational machine responsible for entry into host cells and the sole target of neutralizing antibodies during infection. We utilized an electron-transfer/higher energy collision dissociation ion fragmentation scheme (Frese, C. K. et al., 2013.) in combination with cryo-electron microscopy to resolve the extensive glycan shield that obstructs the protein surface. These glycans provide a structural framework to understanding the accessibility of the protein to antibodies.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Human Coronavirus Nl63

SUBMITTER: Joost Snijder  

LAB HEAD: David Veesler

PROVIDER: PXD004557 | Pride | 2016-09-27

REPOSITORIES: pride

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01_NL63_trypsin_intact.pep.xml Pepxml
01_NL63_trypsin_intact.raw Raw
01_NL63_trypsin_intact.zip Other
02_NL63_chymotrypsin_intact.pep.xml Pepxml
02_NL63_chymotrypsin_intact.raw Raw
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Publications

Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy.

Walls Alexandra C AC   Tortorici M Alejandra MA   Frenz Brandon B   Snijder Joost J   Li Wentao W   Rey Félix A FA   DiMaio Frank F   Bosch Berend-Jan BJ   Veesler David D  

Nature structural & molecular biology 20160912 10

The threat of a major coronavirus pandemic urges the development of strategies to combat these pathogens. Human coronavirus NL63 (HCoV-NL63) is an α-coronavirus that can cause severe lower-respiratory-tract infections requiring hospitalization. We report here the 3.4-Å-resolution cryo-EM reconstruction of the HCoV-NL63 coronavirus spike glycoprotein trimer, which mediates entry into host cells and is the main target of neutralizing antibodies during infection. The map resolves the extensive glyc  ...[more]

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