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Liquid Chromatography Ion Mobility Mass Spectrometry analysis of CPTAC Comparator Reference sample CompRef P6

ABSTRACT: Proteomic discovery and targeted monitoring of proteins in a breast cancer xenograft tissue sample using LC Ion Mobility Mass Spectrometry.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Breast Cancer Cell

DISEASE(S): Breast Cancer

SUBMITTER: Matthew Monroe

LAB HEAD: Richard D. Smith

PROVIDER: PXD004569 | Pride | 2016-08-10

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	CPTAC_LabelFree_P6_1_1Aug14_Pippin_13-04-12.mgf	Mgf
	CPTAC_LabelFree_P6_1_1Aug14_Pippin_13-04-12.pride.mgf.gz	Mgf
	CPTAC_LabelFree_P6_1_1Aug14_Pippin_13-04-12.raw	Raw
	CPTAC_LabelFree_P6_1_1Aug14_Pippin_13-04-12.tsv	Tabular
	CPTAC_LabelFree_P6_1_1Aug14_Pippin_13-04-12_msgfplus.mzid.gz	Mzid

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Publications

Simultaneous Proteomic Discovery and Targeted Monitoring using Liquid Chromatography, Ion Mobility Spectrometry, and Mass Spectrometry.

Burnum-Johnson Kristin E KE Nie Song S Casey Cameron P CP Monroe Matthew E ME Orton Daniel J DJ Ibrahim Yehia M YM Gritsenko Marina A MA Clauss Therese R W TR Shukla Anil K AK Moore Ronald J RJ Purvine Samuel O SO Shi Tujin T Qian Weijun W Liu Tao T Baker Erin S ES Smith Richard D RD

Molecular & cellular proteomics : MCP 20160925 12

Current proteomic approaches include both broad discovery measurements and quantitative targeted analyses. In many cases, discovery measurements are initially used to identify potentially important proteins (e.g. candidate biomarkers) and then targeted studies are employed to quantify a limited number of selected proteins. Both approaches, however, suffer from limitations. Discovery measurements aim to sample the whole proteome but have lower sensitivity, accuracy, and quantitation precision tha ...[more]

PMID: 27670688

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Liquid Chromatography Ion Mobility Mass Spectrometry Analysis of CPTAC Comparator Reference Sample CompRef P6

Project description:Proteomic discovery and targeted monitoring of proteins in a breast cancer xenograft tissue sample using LC Ion Mobility Mass Spectrometry.

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