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Comprehensive analysis of the membrane phosphoproteome regulated by oligogalacturonides in Arabidopsis thaliana

ABSTRACT: Early changes in the Arabidopsis thaliana membrane phosphoproteome in response to oligogalacturonides (OGs), a class of plant damage-associated molecular patterns (DAMPs), were analyzed through phosphopeptide enrichment followed by LC-MS/MS using label-free quantification. This large-scale phosphoproteome analysis of early OG-signaling enabled us to determine 100 regulated phosphosites.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Seedling

SUBMITTER: Benedetta Mattei

LAB HEAD: Benedetta Mattei

PROVIDER: PXD004599 | Pride | 2016-08-22

REPOSITORIES: Pride

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Comprehensive Analysis of the Membrane Phosphoproteome Regulated by Oligogalacturonides in Arabidopsis thaliana.

Mattei Benedetta B Spinelli Francesco F Pontiggia Daniela D De Lorenzo Giulia G

Frontiers in plant science 20160802

Early changes in the Arabidopsis thaliana membrane phosphoproteome in response to oligogalacturonides (OGs), a class of plant damage-associated molecular patterns (DAMPs), were analyzed by two complementary proteomic approaches. Differentially phosphorylated sites were determined through phosphopeptide enrichment followed by LC-MS/MS using label-free quantification; differentially phosphorylated proteins were identified by 2D-DIGE combined with phospho-specific fluorescent staining (phospho-DIGE ...[more]

PMID: 27532006

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