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Nanoparticle protein corona LC-MS/MS

ABSTRACT: Engineered nanoparticles for biomedical applications require increased effectiveness in targeting specific cells while preserving non-target cells’ safety. Native nanoparticles entering a protein-rich liquid media quickly form a macromolecular structure called protein corona. This protein structure defines the physical interaction between nanoparticles and target cells. We describe SUSTU (surface proteomics for nanoparticle safety, targeting and uptake) a proteomics-based method to analyze the surface of a nanoparticle protein corona. This method provides qualitative and quantitative analysis from the protein corona surface. Those exposed proteins compose the first line of interaction between this macromolecular structure and the cell surface of a target cell. With SUSTU, the spatial and temporal dynamics of the protein corona surface can be studied. Data from SUSTU would ascertain the nanoparticle functionalized groups exposed at destiny; circumventing preliminary in vitro experiments. Therefore this method evaluates nanoparticle targeting and uptake capability and could be integrated into a rapid prototyping strategy which is a major challenge in nanomaterial science.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Cell Culture

SUBMITTER: Jacob Kuruvilla

LAB HEAD: Susana Christobal

PROVIDER: PXD004636 | Pride | 2017-02-28

REPOSITORIES: Pride

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