Dataset Information

Proteomic profiling of human cancer pseudopodia for the identification of anti-metastatic drug candidates

ABSTRACT: Cancer metastasis causes approximately 90% of all cancer-related death. Independent of the advancement of cancer therapy, a majority of late stage patients suffers from metastatic cancer. Metastasis progresses by cancer cell migration and invasion throughout the body. This migration of cancer cells requires the formation of pseudopodia in the direction of movement, but detailed understanding of the process and accordingly strategies of prevention remain elusive. Here, we use quantitative proteomic profiling of human cancer pseudopodia to examine this mechanisms essential to metastasis formation, and identify potential candidates for pharmacological interference with the process. We demonstrate that Prohibitins (PHBs) are significantly enriched in the pseudopodia fraction derived from cancer cells, and knockdown of PHBs, as well as their chemical inhibition through Rocaglamide (Roc-A), efficiently reduces cancer cell migration.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Johannes Graumann

LAB HEAD: Johannes Graumann

PROVIDER: PXD004658 | Pride | 2018-04-12

REPOSITORIES: pride

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			Action	DRS
	20151202_QE1_SUC_MDA_CBPD_HL_1_1.raw	Raw
	20151202_QE1_SUC_MDA_CBPD_HL_1_2.raw	Raw
	20151202_QE1_SUC_MDA_CBPD_HL_2_1.raw	Raw
	20151202_QE1_SUC_MDA_CBPD_HL_2_2.raw	Raw
	20151202_QE1_SUC_MDA_CBPD_HL_3_1.raw	Raw

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Proteomic profiling of human cancer pseudopodia for the identification of anti-metastatic drug candidates.

Choi Sunkyu S Bhagwat Aditya M AM Al Mismar Rasha R Goswami Neha N Ben Hamidane Hisham H Sun Lu L Graumann Johannes J

Scientific reports 20180411 1

Cancer metastasis causes approximately 90% of all cancer-related death and independent of the advancement of cancer therapy, a majority of late stage patients suffers from metastatic cancer. Metastasis implies cancer cell migration and invasion throughout the body. Migration requires the formation of pseudopodia in the direction of movement, but a detailed understanding of this process and accordingly strategies of prevention remain elusive. Here, we use quantitative proteomic profiling of human ...[more]

PMID: 29643415

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