Proteomics

Dataset Information

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Saprolegnia parasitica proteome


ABSTRACT: In this project, we made a comprehensive quantitative proteomic analysis of four defined developmental stages of S. parasitica (mycelium, primary cysts, secondary cysts and germinated cysts) to gain greater insight into the types of proteins specifically linked to each stage. A total of 2423 unique proteins were identified using qualitative (gel-based) and quantitative (iTRAQ, isobaric tags for relative and absolute quantitation) approaches. Of these, 358 proteins, associated with various biological processes, were found to be significantly enriched between different life cycle stages of S. parasitica. The transcript abundance of several cyst and mycelium enriched proteins was also checked by quantitative real-time PCR. This is the first large scale proteomic analysis on Saprolegnia and the data from this study will enhance our current knowledge about this pathogen by identifying biological processes key to each developmental stage.

INSTRUMENT(S): Xevo Q-Tof

ORGANISM(S): Oomycetes

TISSUE(S): Cyst

SUBMITTER: Vaibhav Srivastava  

LAB HEAD: Vincent Bulone / Vaibhav Srivastava

PROVIDER: PXD004695 | Pride | 2018-08-10

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
2014LanaGerminatingCystBands.mgf Mgf
2014LanaITRAQBR1100mMa.mgf Mgf
2014LanaITRAQBR1100mMb.mgf Mgf
2014LanaITRAQBR1125mMa.mgf Mgf
2014LanaITRAQBR1125mMb.mgf Mgf
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Publications

Quantitative Proteomic Analysis of Four Developmental Stages of <i>Saprolegnia parasitica</i>.

Srivastava Vaibhav V   Rezinciuc Svetlana S   Bulone Vincent V  

Frontiers in microbiology 20180111


Several water mold species from the <i>Saprolegnia</i> genus infect fish, amphibians, and crustaceans in natural ecosystems and aquaculture farms. <i>Saprolegnia parasitica</i> is one of the most severe fish pathogens. It is responsible for millions of dollars of losses to the aquaculture industry worldwide. Here, we have performed a proteomic analysis, using gel-based and solution (iTRAQ) approaches, of four defined developmental stages of <i>S. parasitica</i> grown <i>in vitro</i>, i.e., the m  ...[more]

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