Proteomics

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ProteomeTools - Building ProteomeTools based on a complete synthetic human proteome

ABSTRACT: The ProteomeTools project aims to derive molecular and digital tools from the human proteome to facilitate biomedical and life science research. Here, we describe the generation and multimodal LC-MS/MS analysis of >350,000 synthetic tryptic peptides representing nearly all canonical human gene products. This resource will be extended to 1.4 million peptides within two years and all data will be made available to the public in ProteomicsDB.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Daniel Zolg  

LAB HEAD: Bernhard Kuster

PROVIDER: PXD004732 | Pride | 2017-02-09

REPOSITORIES: Pride

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01625b_GA1-TUM_first_pool_1_01_01-2xIT_2xHCD-1h-R1.raw Raw
01625b_GA1-TUM_first_pool_1_01_01-3xHCD-1h-R1.raw Raw
01625b_GA1-TUM_first_pool_1_01_01-DDA-1h-R2.raw Raw
01625b_GA1-TUM_first_pool_1_01_01-ETD-1h-R2.raw Raw
01625b_GA2-TUM_first_pool_9_01_01-2xIT_2xHCD-1h-R1.raw Raw
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We describe ProteomeTools, a project building molecular and digital tools from the human proteome to facilitate biomedical research. Here we report the generation and multimodal liquid chromatography-tandem mass spectrometry analysis of >330,000 synthetic tryptic peptides representing essentially all canonical human gene products, and we exemplify the utility of these data in several applications. The resource (available at http://www.proteometools.org) will be extended to >1 million peptides, a  ...[more]

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