Dataset Information

Analysis of the yeast peptidome and comparison with the human peptidome

ABSTRACT: Peptides function as signaling molecules in species as diverse as humans and yeast. Mass spectrometry-based peptidomics techniques provide a relatively unbiased method to assess the peptidome of biological samples. In the present study, we used a quantitative peptidomic technique to characterize the peptidome of the yeast Saccharomyces cerevisiae and compare it to the peptidomes of mammalian cell lines and tissues. Altogether, 297 yeast peptides derived from 75 proteins were identified. The yeast peptides are similar to those of the human peptidome in average size and amino acid composition. Inhibition of proteasome activity with either bortezomib or epoxomicin led to decreased levels of some yeast peptides, suggesting that these peptides are generated by the proteasome. Approximately 30% of the yeast peptides correspond to the N- or C-terminus of the protein; the human peptidome is also highly represented in N- or C-terminal protein fragments. Most yeast and humans peptides are derived from a subset of abundant proteins, many with functions involving cellular metabolism or protein synthesis and folding. Of the 75 yeast proteins that give rise to peptides, 24 have orthologs that give rise to human and/or mouse peptides and for some, the same region of the proteins are found in the human, mouse, and yeast peptidomes. Taken together, these results support the hypothesis that intracellular peptides may have specific and conserved biological functions.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Alexandre Keiji Tashima

LAB HEAD: Lloyd D. Fricker

PROVIDER: PXD004800 | Pride | 2020-06-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20140718_Leandro_Yeast_WT_001.raw.zip	Raw
	20140718_Leandro_Yeast_WT_001_CID.pkl	Other
	20140718_Leandro_Yeast_bortez_001.raw.zip	Raw
	20140718_Leandro_Yeast_bortez_001_CID.pkl	Other
	20141124_pdr5del_BOR.raw.zip	Raw

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Publications

Analysis of the Yeast Peptidome and Comparison with the Human Peptidome.

Dasgupta Sayani S Yang Ciyu C Castro Leandro M LM Tashima Alexandre K AK Ferro Emer S ES Moir Robyn D RD Willis Ian M IM Fricker Lloyd D LD

PloS one 20160929 9

Peptides function as signaling molecules in species as diverse as humans and yeast. Mass spectrometry-based peptidomics techniques provide a relatively unbiased method to assess the peptidome of biological samples. In the present study, we used a quantitative peptidomic technique to characterize the peptidome of the yeast Saccharomyces cerevisiae and compare it to the peptidomes of mammalian cell lines and tissues. Altogether, 297 yeast peptides derived from 75 proteins were identified. The yeas ...[more]

PMID: 27685651

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