Dataset Information

Monitoring of protein thiol-oxidation in S.aureus USA300 under hypochlorite stress using OxICAT approach.

ABSTRACT: During infections, S. aureus has to cope with the oxidative burst of activated macrophages and neutrophils, including reactive oxygen and nitrogen species (RNS, ROS) and the strong oxidant hypochloric acid. We aimed to understand the global thiol-redox state in the major pathogen S. aureus and discover new NaOCl-sensitive proteins driven by thiol-switches. Thus, we performed a quantitative redox proteomics approach based on OxICAT and analyzed the percentages of thiol-oxidation levels in S.aureus before and after sub-lethal doses of 150 µM NaOCl stress. In parallel, we searched for protein S-bacillithiolation.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Marcel Imber

LAB HEAD: Haike Antelmann

PROVIDER: PXD004918 | Pride | 2016-12-22

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	140131_V3_P5_CH_HA_NaOCl_1-1.raw	Raw
	140131_V3_P5_CH_HA_NaOCl_1-2.raw	Raw
	140131_V3_P5_CH_HA_NaOCl_1-3.raw	Raw
	140131_V3_P5_CH_HA_NaOCl_1-4.raw	Raw
	140131_V3_P5_CH_HA_NaOCl_1-5.raw	Raw

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Publications

Protein S-Bacillithiolation Functions in Thiol Protection and Redox Regulation of the Glyceraldehyde-3-Phosphate Dehydrogenase Gap in Staphylococcus aureus Under Hypochlorite Stress.

Imber Marcel M Huyen Nguyen Thi Thu NTT Pietrzyk-Brzezinska Agnieszka J AJ Loi Vu Van VV Hillion Melanie M Bernhardt Jörg J Thärichen Lena L Kolšek Katra K Saleh Malek M Hamilton Chris J CJ Adrian Lorenz L Gräter Frauke F Wahl Markus C MC Antelmann Haike H

Antioxidants & redox signaling 20170118 6

<h4>Aims</h4>Bacillithiol (BSH) is the major low-molecular-weight thiol of the human pathogen Staphylococcus aureus. In this study, we used OxICAT and Voronoi redox treemaps to quantify hypochlorite-sensitive protein thiols in S. aureus USA300 and analyzed the role of BSH in protein S-bacillithiolation.<h4>Results</h4>The OxICAT analyses enabled the quantification of 228 Cys residues in the redox proteome of S. aureus USA300. Hypochlorite stress resulted in >10% increased oxidation of 58 Cys res ...[more]

PMID: 27967218

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