Dataset Information

Proteomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in Synechocystis sp. PCC 6803

ABSTRACT: Using an engineered 3-HP producing SM strain, in this project, the cellular responses to 3-HP internally produced were first determined using a quantitative iTRAQ-LC-MS/MS proteomics approach. A total of 2,264 unique proteins were identified, which represented about 63% of all predicted protein in Synechocystis in the proteomic analysis of the 3-HP producing strain SM and wild type. Among all identified proteins, 204 proteins were found up- regulated and 123 proteins were found down-regulated, respectively. The proteins related to oxidative phosphorylation, photosynthesis, ribosome, central carbon metabolism, two-component systems and ABC-type transporters were up-regulated. The results suggested that the supply of ATP and NADPH was increased significantly, and the precursor malonyl-CoA and acetyl-CoA may also be supplemented when 3-HP was produced at a high level in Synechocystis.

INSTRUMENT(S): 4700 Proteomics Analyzer

ORGANISM(S): Synechococcus Cyanophage Syn

SUBMITTER: Yunpeng Wang

LAB HEAD: Weiwen Zhang

PROVIDER: PXD004974 | Pride | 2016-09-26

REPOSITORIES: Pride

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Proteomic and metabolomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in cyanobacterium <i>Synechocystis</i> sp. PCC 6803.

Wang Yunpeng Y Chen Lei L Zhang Weiwen W

Biotechnology for biofuels 20161006

<h4>Background</h4>3-hydroxypropionic acid (3-HP) is an important platform chemical with a wide range of applications. In our previous study, the biosynthetic pathway of 3-HP was constructed and optimized in cyanobacterium <i>Synechocystis</i> sp. PCC 6803, which led to 3-HP production directly from CO<sub>2</sub> at a level of 837.18 mg L<sup>-1</sup> (348.8 mg/g dry cell weight). As the production and accumulation of 3-HP in cells affect cellular metabolism, a better understanding of cellular ...[more]

PMID: 27757169

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