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AP-MS of EPOP in mouse embryonic stem cells

ABSTRACT: A Flag-tagged version of murine EPOP was expressed in mouse embryonic stem cells (ESCs) at near to endogenous levels and purified using an anti-FLAG affinity matrix.

OTHER RELATED OMICS DATASETS IN: PRJNA316432

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryo, Embryonic Stem Cell

SUBMITTER: Michael Wierer

LAB HEAD: Luciano di Croce

PROVIDER: PXD005082 | Pride | 2016-12-13

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	1203014_MWMB_FLAG_1.raw	Raw
	1203014_MWMB_FLAG_2.raw	Raw
	1203014_MWMB_FLAG_3.raw	Raw
	1203014_MWMB_PRC3_1.raw	Raw
	1203014_MWMB_PRC3_2.raw	Raw

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1 - 5 of 7

Publications

EPOP Functionally Links Elongin and Polycomb in Pluripotent Stem Cells.

Beringer Malte M Pisano Paola P Di Carlo Valerio V Blanco Enrique E Chammas Paul P Vizán Pedro P Gutiérrez Arantxa A Aranda Sergi S Payer Bernhard B Wierer Michael M Di Croce Luciano L

Molecular cell 20161101 4

The cellular plasticity of pluripotent stem cells is thought to be sustained by genomic regions that display both active and repressive chromatin properties. These regions exhibit low levels of gene expression, yet the mechanisms controlling these levels remain unknown. Here, we describe Elongin BC as a binding factor at the promoters of bivalent sites. Biochemical and genome-wide analyses show that Elongin BC is associated with Polycomb Repressive Complex 2 (PRC2) in pluripotent stem cells. Elo ...[more]

PMID: 27863225

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AP-MS of Elongin B in mouse embryonic stem cells

Project description:A Flag-tagged version of murine Elongin B was expressed in mouse embryonic stem cells (ESCs) at near to endogenous levels and purified using an anti-FLAG affinity matrix.

2016-12-13 | PXD005081 | Pride

The evolutionary impact of Intragenic FliA Promoters

Project description:We looked at binding sites in a FliA FLAG-tagged wild-type Salmonella enterica serovar Typhimurium strain.

2017-09-18 | E-MTAB-6049 | biostudies-arrayexpress

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2019-10-04 | E-MTAB-8370 | biostudies-arrayexpress

ChIP-seq of E. coli to identify H-NS and Fis binding at multiple time-points during growth in rich medium

Project description:Among the most important regulators of gene expression in bacteria are 'nucleoid-associated proteins'. These proteins alter the topology of the bound DNA by bending, wrapping or bridging it, thus having multiple effects, including transcriptional regulation, on the bacterial cell. Among the best-studied nucleoid proteins are H-NS and Fis, which bind to specific sequences on the DNA. H-NS is a global repressor of gene expression. Fis alters the global conformation of the DNA by introducing branched structures in it; but its effect on gene expression on a genomic scale remains largely unclear.<br><br>Several bacterial transcriptional regulators including H-NS and Fis have been studied using ChIP-chip. However, the higher resolution and dynamic range offered by ChIP-Seq have not been exploited for any bacterial species. By performing ChIP-Seq of these two proteins, we present the first such study in a bacterium. In addition to providing a proof-of-principle for the use of this technology for bacteria, we perform our study at multiple time-points during growth in rich medium, thus generating new insights into how these proteins function under different cellular conditions. Further, by analysing our data in conjunction with newly-generated gene expression and RNA polymerase-chromosome interaction data we provide new interpretation of the genome-scale patterns of the interactions of these proteins to the DNA.

2010-09-01 | E-MTAB-332 | biostudies-arrayexpress

GBA1 interactome for understanding pathological mechanisms

Project description:Compound heterozygous or homozygous GBA1 mutations lead to Gaucher disease. Furthermore, GBA1 mutations are the most frequent risk factor for Parkinsonâ€™s disease. To get a better understanding of the pathological mechanisms, we generated inducible V5-Flag-Tag, V5-Flag-tagged WT, E326K and L444P mutant Flp-Inâ„¢T-RExâ„¢-HEK293 and performed interatomic analysis.

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Salmonella 14028s AraC ChIP-Seq

Project description:We mapped the genome-wide binding of C-terminally FLAG-tagged AraC in S. enterica subsp. enterica serovar Typhimurium strain 14028s using ChIP coupled with deep sequencing (ChIP-seq). We identified five putative target loci for AraC: upstream of araB/araC, araE, araJ, STM14_0178, and within sseD.

2013-10-09 | E-MTAB-1915 | biostudies-arrayexpress

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Project description:We mapped the genome-wide binding of the flagellar regulators FlhD, FlhC, and FliA in FLAG-tagged derivatives of E. coli K-12 MG1655 using ChIP coupled with deep sequencing (ChIP-seq). We identify new binding sites for each factor.

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AopACT binding in adult gut and fat body upon its induction in Drosophila melanogaster

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The RPB2 flap loop of human RNA polymerase II is dispensable for transcription initiation and elongation.

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ChIP-seq experiment of SMC-FLAG binding to Streptomyces venezuelae chromosome in wild type and parB knockout strain

Project description:We studied the binding of SMC protein to Streptomyces venezuelae chromosome during development of aerial hyphae (14 hour of growth) using SMC-FLAG protein. Additionally investigated the role of ParB protein in SMC DNA binding using parB deletion strain.

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