Dataset Information

Longitudinal study of the small urinary extracellular vesicle proteome during living donor kidney transplantation

ABSTRACT: Urine was obtained from healthy kidney donors, from transplanted kidneys intrasurgery, at postoperative day 1 and at later timepoints. Exosomes were isolated and measured using nLC-MS/MS. For details, see the accompanying manuscript.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine

SUBMITTER: Markus Rinschen

LAB HEAD: Roman-Ulrich Mueller

PROVIDER: PXD005219 | Pride | 2021-08-30

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	150722_submission_FaBr_exosome.doc	Other
	20150901_UC_Braun_Benzing_0138_150722FaBrS01.raw	Raw
	20150901_UC_Braun_Benzing_0138_150722FaBrS02.raw	Raw
	20150901_UC_Braun_Benzing_0138_150722FaBrS03.raw	Raw
	20150901_UC_Braun_Benzing_0138_150722FaBrS04.raw	Raw

Items per page:

1 - 5 of 92

Publications

The proteomic landscape of small urinary extracellular vesicles during kidney transplantation.

Braun Fabian F Rinschen Markus M Buchner Denise D Bohl Katrin K Plagmann Ingo I Bachurski Daniel D Richard Späth Martin M Antczak Philipp P Göbel Heike H Klein Corinna C Lackmann Jan-Wilm JW Kretz Oliver O Puelles Victor G VG Wahba Roger R Hallek Michael M Schermer Bernhard B Benzing Thomas T Huber Tobias B TB Beyer Andreas A Stippel Dirk D Kurschat Christine E CE Müller Roman-Ulrich RU

Journal of extracellular vesicles 20201001 1

Kidney transplantation is the preferred renal replacement therapy available. Yet, long-term transplant survival is unsatisfactory, partially due to insufficient possibilities of longitudinal monitoring and understanding of the biological processes after transplantation. Small urinary extracellular vesicles (suEVs) - as a non-invasive source of information - were collected from 22 living donors and recipients. Unbiased proteomic analysis revealed temporal patterns of suEV protein signature and ce ...[more]

PMID: 33304478

Similar Datasets

Project description:Adult-to-adult living donors and recipients were studied to characterize patterns of liver growth and identify associated factors in a multicenter study. Three hundred and fifty donors and 353 recipients in the Adult-to-Adult Living Donor Liver Transplantation Cohort Study (A2ALL) receiving transplants between March 2003 and February 2010 were included. Potential predictors of 3-month liver volume included total and standard liver volumes (TLV and SLV), Model for End-Stage Liver Disease (MELD) score (in recipients), the remnant and graft size, remnant-to-donor and graft-to-recipient weight ratios (RDWR and GRWR), remnant/TLV, and graft/SLV. Among donors, 3-month absolute growth was 676 ± 251 g (mean ± SD), and percentage reconstitution was 80% ± 13%. Among recipients, GRWR was 1.3% ± 0.4% (8 < 0.8%). Graft weight was 60% ± 13% of SLV. Three-month absolute growth was 549 ± 267 g, and percentage reconstitution was 93% ± 18%. Predictors of greater 3-month liver volume included larger patient size (donors and recipients), larger graft volume (recipients), and larger TLV (donors). Donors with the smallest remnant/TLV ratios had larger than expected growth but also had higher postoperative bilirubin and international normalized ratio at 7 and 30 days. In a combined donor-recipient analysis, donors had smaller 3-month liver volumes than recipients adjusted for patient size, remnant or graft volume, and TLV or SLV (P = 0.004). Recipient graft failure in the first 90 days was predicted by poor graft function at day 7 (HR = 4.50, P = 0.001) but not by GRWR or graft fraction (P > 0.90 for each). Both donors and recipients had rapid yet incomplete restoration of tissue mass in the first 3 months, and this confirmed previous reports. Recipients achieved a greater percentage of expected total volume. Patient size and recipient graft volume significantly influenced 3-month volumes. Importantly, donor liver volume is a critical predictor of the rate of regeneration, and donor remnant fraction affects postresection function. Liver Transpl 21:79-88, 2015. © 2014 AASLD.