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Tapered-tip Capillary Electrophoresis Nano-electrospray Ionization Mass Spectrometry for Ultrasensitive Proteomics: The Mouse Cortex

ABSTRACT: We have enabled ultrasensitive proteomics of limited protein digests by developing a tapered-tip nanoelectrospray ionization (nanoESI) interface for microanalytical capillary electrophoresis (CE) high resolution mass spectrometry (HRMS). CE-nanoESI-HRMS allowed us to detect 260 zmol angiotensin II (lower limit of detection) and identify 1 pg of bovine serum albumin and cytochrome c. A 1 ng protein digest from the mouse posterior cortex yielded 217 nonredundant protein groups, including many gene products that are used as classical neuronal markers.

INSTRUMENT(S): maXis

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Tissue, Brain

SUBMITTER: Peter Nemes

LAB HEAD: Peter Nemes

PROVIDER: PXD005222 | Pride | 2022-02-18

REPOSITORIES: Pride

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			Action	DRS
	2015-12-15_SC03NoDMSOTechnReplicate1.d.zip	Other
	2015-12-15_SC03NoDMSOTechnReplicate1MaxQuantSEARCH.zip	Other
	2015-12-15_SC04NoDMSOTechnReplicate2.d.zip	Other
	2015-12-15_SC04NoDMSOTechnReplicate2MaxQuantSEARCH.zip	Other
	2015-12-15_SC05NoDMSOTechnReplicate3.d.zip	Other

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