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The 15-min Subcellular Proteome

ABSTRACT: This project integrated data-independent acquisition (DIA) with capillary electrophoresis electrospray ionization mass spectrometry (CE-ESI-MS) to enable fast single-cell proteomics. With <15 min of effective separation time in a bottom-up proteomics setting, this technology returned ~1,200 proteins from a single HeLa-cell-equivalent proteome amount. Furthermore, using capillary microsampling to collect the proteome from identified cells in a cleavage-stage embryo of the vertebrate Xenopus laevis (frog) embryo, ~1,400 proteins were identified by measuring ~5 ng of the subcellular proteome content aspirated from live Xenopus embryos. CE-ESI-MS with DIA enhanced proteome detection sensitivity and improved analytical throughput.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Xenopus Laevis (african Clawed Frog)

TISSUE(S): Embryo

SUBMITTER: Peter Nemes

LAB HEAD: Peter Nemes

PROVIDER: PXD046467 | Pride | 2024-09-27

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	10X40_200pgHeLa_report.pg_matrix.tsv	Tabular
	2020_12_17_BS01_hela_DDA_90min_trial1.RAW	Raw
	2020_12_17_BS01_heladigest_350-900_trial1.RAW	Raw
	2020_12_21_BS01_hela_DDA_90min_trial2.RAW	Raw
	2020_12_21_BS01_heladigest_350-900_trial2.RAW	Raw

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