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On tissue Top down microproteomic bridged to MALDI MS Imaging reveal the molecular physiome of brain regions

ABSTRACT: Tissue Top-down microproteomic was performed on 3 brain regions. 156 references proteins from different cellular compartments were characterized. Moreover, 11 Alternative proteins issued from alternative open reading frames (AltORF) were identified and were relied to the brain regions and associated to the function of their reference proteins. Some proteins display specific post-translational modifications profiles or truncation linked the brain regions and their functions. Systemic biology performed on microproteome identified in each region allowed to associate sub-networks with functional physiology of each brain region. Back correlation of the local extracted and identified microproteome with tissue cellular localization was then performed by MALDI mass spectrometry imaging. 40 proteins including 4 Altproteins have been back correlated in MALDI MSI and are in line with their tissue extracted region and physiological function. Taken together, we established the molecular physiome of 3 rat brain regions through reference and hidden proteome characterization.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Julien Franck

LAB HEAD: Michel Salzet

PROVIDER: PXD005424 | Pride | 2017-11-14

REPOSITORIES: Pride

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Spatially-Resolved Top-down Proteomics Bridged to MALDI MS Imaging Reveals the Molecular Physiome of Brain Regions.

Delcourt Vivian V Franck Julien J Quanico Jusal J Gimeno Jean-Pascal JP Wisztorski Maxence M Raffo-Romero Antonella A Kobeissy Firas F Roucou Xavier X Salzet Michel M Fournier Isabelle I

Molecular & cellular proteomics : MCP 20171109 2

Tissue spatially-resolved proteomics was performed on 3 brain regions, leading to the characterization of 123 reference proteins. Moreover, 8 alternative proteins from alternative open reading frames (AltORF) were identified. Some proteins display specific post-translational modification profiles or truncation linked to the brain regions and their functions. Systems biology analysis performed on the proteome identified in each region allowed to associate sub-networks with the functional physiolo ...[more]

PMID: 29122912

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